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Key Documents

S6402

Sigma-Aldrich

Streptavidin−Cy3 from Streptomyces avidinii

buffered aqueous solution

Sinônimo(s):

Streptavidin Cy3

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.46

conjugado

CY3 conjugate

Nível de qualidade

forma

buffered aqueous solution

Extensão da rotulagem

3-9 mol fluorochrome per mol protein

técnica(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

Condições de expedição

wet ice

temperatura de armazenamento

2-8°C

Descrição geral

Streptavidin is a homotetramer protein. One molecule of streptavidin binds four molecules of biotin by a non-covalent interaction that is essentially irreversible. Streptavidin has a high affinity for biotin and is extensively used as a labeling tool and for studies involving binding proteins.

Aplicação

Streptavidin−Cy3 from Streptomyces avidinii has been used:
  • in double immunofluorescence staining to detect biotinylated lectin
  • for insulin and C-peptide staining in immunofluorescence analysis
  • in double immunofluorescence to verify the presence of cells filled with biocytin
  • in conjunction with biotinylated antibodies in immunohistochemistry and immunocytochemistry
  • with the traditional rhodamine filter arrangements due to the spectral overlap

Características e benefícios

Spectral Characteristics of Cy3 Dye:
  • Absorbance Max 552 nm
  • Emission Max 565 nm
  • Molar Extinction Coefficient 1.3 x 10-5 M-1cm-1

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 15 mM sodium azide

Informações legais

Cy3 is a trademark of Cytiva

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Artigos

Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.

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