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Key Documents

40709

Sigma-Aldrich

Atto 590-Streptavidin

BioReagent, suitable for fluorescence

Sinônimo(s):

Streptavidin-Atto 590

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About This Item

Número MDL:
Código UNSPSC:
12352108
NACRES:
NA.32

linha de produto

BioReagent

Nível de qualidade

forma

powder

fabricante/nome comercial

ATTO-TEC GmbH

fluorescência

λex 590 nm; λem 619 nm in 0.1 M phosphate pH 7.0

adequação

suitable for fluorescence

temperatura de armazenamento

−20°C

Aplicação

Atto 590 is a new label with high molecular absorption (120.000) and quantum yield (0.80) as well as sufficient stoke′s shift (excitation maximum 594 nm, emission maximum 624 nm). Atto 590 has been coupled to streptavidin to enable all kinds of assays based on the specific affinity of biotin to streptavidin. Streptavidin conjugates are used as secondary detection reagents in histochemical applications, flow cytometry, microarrays, blot analysis and immunoassays. Streptavidin conjugation of Atto 590 does not significantly change the labels spectral data regarding excitation and emission maxima. Given the optimized Dye/Protein-ratio molar absorbance and quantum yields remain high within application. Conjugates of our Atto dyes generally do not show strong fluorescence in unbound state, but fluorescence is boosted again as soon as they are bound to biotin. This may help overcome problems by unspecific binding which may sometimes appear with streptavidin based assays in special cases.

Nota de análise

free of unconjugated dye

Informações legais

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)


Certificados de análise (COA)

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Nano letters, 10(6), 2192-2196 (2010-05-21)
Semiconductor quantum dots (QDs) are used for the optical analysis of casein kinase (CK2) or the hydrolytic activity of alkaline phosphatase (ALP). Two schemes for the analysis of CK2 by a FRET-based mechanism are described. One approach involves the CK2-catalyzed
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Artigos

Fluorescent Labeling of Peptides

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Atto dyes are a series of fluorescent dyes that meet the critical needs of modern fluorescent technologies

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