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Sigma-Aldrich

Atto 647N-Streptavidin

BioReagent, suitable for fluorescence

Sinônimo(s):

Streptavidin-Atto 647N

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About This Item

Código UNSPSC:
12161900
NACRES:
NA.25

linha de produto

BioReagent

Nível de qualidade

100

forma

powder

fabricante/nome comercial

ATTO-TEC GmbH

λ

in ethanol (with 0.1% trifluoroacetic acid)

Absorção UV

λ: 650.0-656.0 nm Amax

adequação

corresponds for gel electrophoresis
suitable for fluorescence

temperatura de armazenamento

−20°C

Descrição geral

Atto 647N is a new generation fluorescent dye dedicated towards the red spectral region. It is a cationic dye and carries a net electrical charge of +1 post substrate coupling reaction. Streptavidin, isolated from Streptomyces avidinii, is a tetrameric protein of 4 x 13.2 kDa which binds very tightly to the small molecule biotin. The dissociation constant of the complex is extremely small (Kd ≈ 10-15 M), ranking among the strongest non-covalent interactions. Atto streptavidin conjugates are readily soluble in water.

Aplicação

Atto 647N-Streptavidin is designed for application in the area of life science, e.g., labeling of DNA, RNA or proteins. Atto streptavidin conjugates may be used as secondary detection reagents in flow cytometry, immunoassays, blot analysis, and histochemical applications. Atto 647N -Streptavidin is suitable for multiplex analysis, including multicolor imaging.

Características e benefícios

Atto 647N- Streptavidin has the following benefits-

  • Highly photostable. Strong absorption.
  • Excellent fluorescent quantum yield.
  • Highly resistant to ozone degradation.
  • Minimal triplet formation.

Nota de análise

dye-to-protein ratio ≥1.5

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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