94149
Atto 647N-Streptavidin
BioReagent, suitable for fluorescence
Sinônimo(s):
Streptavidin-Atto 647N
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About This Item
Código UNSPSC:
12161900
NACRES:
NA.25
Produtos recomendados
linha de produto
BioReagent
Formulário
powder
fabricante/nome comercial
ATTO-TEC GmbH
λ
in ethanol (with 0.1% trifluoroacetic acid)
Absorção UV
λ: 650.0-656.0 nm Amax
adequação
corresponds for gel electrophoresis
suitable for fluorescence
temperatura de armazenamento
−20°C
Descrição geral
Atto 647N is a new generation fluorescent dye dedicated towards the red spectral region. It is a cationic dye and carries a net electrical charge of +1 post substrate coupling reaction. Streptavidin, isolated from Streptomyces avidinii, is a tetrameric protein of 4 x 13.2 kDa which binds very tightly to the small molecule biotin. The dissociation constant of the complex is extremely small (Kd ≈ 10-15 M), ranking among the strongest non-covalent interactions. Atto streptavidin conjugates are readily soluble in water.
Aplicação
Atto 647N-Streptavidin is designed for application in the area of life science, e.g., labeling of DNA, RNA or proteins. Atto streptavidin conjugates may be used as secondary detection reagents in flow cytometry, immunoassays, blot analysis, and histochemical applications. Atto 647N -Streptavidin is suitable for multiplex analysis, including multicolor imaging.
Características e benefícios
Atto 647N- Streptavidin has the following benefits-
- Highly photostable. Strong absorption.
- Excellent fluorescent quantum yield.
- Highly resistant to ozone degradation.
- Minimal triplet formation.
Nota de análise
dye-to-protein ratio ≥1.5
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
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Conteúdo relacionado
Atto dyes are a series of fluorescent dyes that meet the critical needs of modern fluorescent technologies
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