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Sigma-Aldrich

Atto 647N NHS ester

BioReagent, suitable for fluorescence, ≥90% (HPLC)

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About This Item

Número MDL:
Código UNSPSC:
12352108
NACRES:
NA.32

linha de produto

BioReagent

Ensaio

≥90% (HPLC)
≥90% (degree of coupling)

forma

solid

fabricante/nome comercial

ATTO-TEC GmbH

λ

in ethanol (with 0.1% trifluoroacetic acid)

Absorção UV

λ: 641-647 nm Amax

adequação

suitable for fluorescence

temperatura de armazenamento

−20°C

Descrição geral

Atto 647N is a superior red-emitting fluorescence dye with a strong absorption, excellent fluorescence quantum yield (65%), high photostability, excellent ozone resistance, good solubility, and very little triplet formation.

Aplicação

Atto 647N belongs to a new generation of fluorescent labels for the red spectral region. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. Characteristic features of the label are strong absorption, excellent fluorescence quantum yield, high photostability, excellent ozone resistance, good solubility, and very little triplet formation. Atto 647N is a cationic dye. The active ester of Atto 647N fluorescence dye reacts with amino groups under mild conditions. After coupling to a substrate the dye carries a net electrical charge of +1. In common with most Atto-labels, absorption and fluorescence are independent of pH in the range of 2 to 11, used in typical applications. As supplied Atto 647N consists of a mixture of two isomers with practically identical absorption and fluorescence properties.

Informações legais

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)


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