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76245

Sigma-Aldrich

Atto 655 NHS ester

BioReagent, suitable for fluorescence

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About This Item

Número MDL:
MFCD03456137
Código UNSPSC:
12352108
ID de substância PubChem:
93577785
NACRES:
NA.32

linha de produto

BioReagent

Nível de qualidade

100

Ensaio

≥90.0% (HPLC)

Formulário

powder

fabricante/nome comercial

ATTO-TEC GmbH

Extensão da rotulagem

≥90.0%

λ

in ethanol (with 0.1% trifluoroacetic acid)

Absorção UV

λ: 650.0-656.0 nm Amax

adequação

suitable for coupling to amines
suitable for fluorescence

temperatura de armazenamento

−20°C

cadeia de caracteres SMILES

[S](=O)(=O)([O-])CC1CC([N+](=C3C1=CC4=Nc5c(cc6c(c5)CCCN6CC)OC4=C3)CCCC(=O)ON2C(=O)CCC2=O)(C)C

InChI

1S/C31H36N4O8S/c1-4-33-11-5-7-19-13-22-26(15-24(19)33)42-27-16-25-21(14-23(27)32-22)20(18-44(39,40)41)17-31(2,3)34(25)12-6-8-30(38)43-35-28(36)9-10-29(35)37/h13-16,20H,4-12,17-18H2,1-3H3

chave InChI

SPDKWNQUMMQOKX-UHFFFAOYSA-N

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Aplicação

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.

Informações legais

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

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Certificados de análise (COA)

Lot/Batch Number
BCCK5539
BCCK0548
BCCJ0102
BCCH8476
BCCG9693

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Fluorescent-labeled DNA is generated through enzymatic incorporation of fluorophore-linked 2'-deoxyribonucleoside-5'-triphosphates (dNTPs) by DNA polymerases. We describe the synthesis of a variety of dye-labeled dNTPs. Amino-linker-modified 5'-triphosphates of all four naturally occurring nucleobases were used as precursors. Commercially available dyes were
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The modification of nucleic acids using nucleotides linked to detectable reporter or functional groups is an important experimental tool in modern molecular biology. This enhances DNA or RNA detection as well as expanding the catalytic repertoire of nucleic acids. Here
Highly sensitive protease assay using fluorescence quenching of peptide probes based on photoinduced electron transfer.
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Steady-state and time-resolved fluorescence measurements were performed to elucidate the fluorescence quenching of oxazine, rhodamine, carbocyanine, and bora-diaza-indacene dyes by amino acids. Among the natural amino acids, tryptophan exhibits the most pronounced quenching efficiency. Especially, the red-absorbing dyes ATTO 655
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