11207741910
Roche
Anti-Digoxigenin-Fluorescein, Fab fragments
from sheep
Sinônimo(s):
anti-digoxigenin, digoxigenin
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About This Item
Código UNSPSC:
12352203
Produtos recomendados
fonte biológica
sheep
Nível de qualidade
conjugado
fluorescein conjugate
forma do anticorpo
purified immunoglobulin
tipo de produto de anticorpo
primary antibodies
clone
polyclonal
forma
lyophilized
embalagem
pkg of 200 μg
fabricante/nome comercial
Roche
Isotipo
IgG
temperatura de armazenamento
2-8°C
Descrição geral
Anti-Digoxigenin-Fluorescein, Fab fragments has fab fragments from polyclonal anti-digoxigenin antibodies, conjugated to fluorescein.
Especificidade
The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.
Aplicação
Anti-Digoxigenin-Fluorescein, Fab fragments are used for the detection of digoxigenin-labeled compounds using:
- Digoxigenin-labeled sugars in glycoconjugate research
- Fluorescent in situ hybridization (FISH)
- Immunohistocytochemistry
- In situ hybridization
Nota de preparo
- Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline:Detection of digoxigenin-labeled sugars in glycoproteins: 50 to 20μg/ml
- Fluorescent in situ hybridization (FISH): 1 to 20μg/ml
- Immunohistocytochemistry: 20 to 50μg/ml
- In situ hybridization: 20 to 50μg/ml
The anti-DIG anitbody is highly specific and sensitive. Therefore, for FISH, a concentration of 1μg/ml is sufficient, and only in case of critical probes, this concentration may be raised to <10μg/ml (with a distinct background to be expected).
Working solution: Reconstitution
Add 1 ml double-distilled water to a final concentration of 200μg/ml.
Blocking:
PBS, 0.5% bovine serum albumin (w/v), pH 7.4.
1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding. Furthermore, pH can be increased up to pH 8.5 to 9.0.
Storage conditions (working solution): Always prepare fresh!
After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption. The Fab fragments obtained by papain digestion were purified by gel filtration, conjugated to the specific label, and stabilized in buffer.
Reconstituição
Add 1 ml double-distilled water to a final concentration of 200 μg/ml.
Outras notas
For life science research only. Not for use in diagnostic procedures.
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Palavra indicadora
Warning
Frases de perigo
Declarações de precaução
Classificações de perigo
Aquatic Chronic 3 - Skin Sens. 1
Código de classe de armazenamento
13 - Non Combustible Solids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
does not flash
Ponto de fulgor (°C)
does not flash
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Artigos
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
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