S2890
Streptavidin−Alkaline Phosphatase from Streptomyces avidinii
lyophilized powder, ≥700 DEA units/mg protein
Sinônimo(s):
Streptavidin-AP, Streptavidin−AP from Streptomyces avidinii
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About This Item
Produtos recomendados
conjugado
alkaline phosphatase conjugate
Nível de qualidade
Formulário
lyophilized powder
atividade específica
≥700 DEA units/mg protein
composição
Protein, ≥40% biuret
temperatura de armazenamento
−20°C
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Descrição geral
Streptavidin, a 58 kDa protein, is a homotetramer purified from Streptomyces avidinii. It has a high affinity for biotin. Streptavidin has been conjugated with either peroxidase or alkaline phosphatase (ALP). These enzymes are used as protein conjugates because of their comparative stability and a wide range of suitable substrates for different applications. ALP conjugates to streptavidin in immunoassays.
Aplicação
Streptavidin-alkaline phosphatase from Streptomyces avidinii has been used in enzyme-linked immune absorbent spot (ELISpot) to detect interferon (IFN-γ) producing cells.
The optimal working dilution should be determined empirically using a range of dilutions from a 1 mg/ml stock in buffer.
Definição da unidade
One alkaline phosphatase unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 9.8 at 37 °C.
forma física
Lyophilized powder containing trehalose with EPPS, phosphate and traces of magnesium chloride and zinc chloride.
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
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Protocolos
Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)
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