Pular para o conteúdo
Merck
Todas as fotos(1)

Documentos Principais

Visualizar Toda a Documentação

S2890

Sigma-Aldrich

Streptavidin−Alkaline Phosphatase from Streptomyces avidinii

lyophilized powder, ≥700 DEA units/mg protein

Sinônimo(s):

Streptavidin-AP, Streptavidin−AP from Streptomyces avidinii

Faça loginpara ver os preços organizacionais e de contrato
Todas as fotos(1)

About This Item

Número MDL:
MFCD00132384
Código UNSPSC:
12352203
NACRES:
NA.46

conjugado

alkaline phosphatase conjugate

Nível de qualidade

200

forma

lyophilized powder

atividade específica

≥700 DEA units/mg protein

composição

Protein, ≥40% biuret

temperatura de armazenamento

−20°C

Procurando produtos similares? Visita Guia de comparação de produtos

Descrição geral

Streptavidin, a 58 kDa protein, is a homotetramer purified from Streptomyces avidinii. It has a high affinity for biotin. Streptavidin has been conjugated with either peroxidase or alkaline phosphatase (ALP). These enzymes are used as protein conjugates because of their comparative stability and a wide range of suitable substrates for different applications. ALP conjugates to streptavidin in immunoassays.

Aplicação

Streptavidin-alkaline phosphatase from Streptomyces avidinii has been used in enzyme-linked immune absorbent spot (ELISpot) to detect interferon (IFN-γ) producing cells.
The optimal working dilution should be determined empirically using a range of dilutions from a 1 mg/ml stock in buffer.

Definição da unidade

One alkaline phosphatase unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 9.8 at 37 °C.

forma física

Lyophilized powder containing trehalose with EPPS, phosphate and traces of magnesium chloride and zinc chloride.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Matured tolerogenic dendritic cells effectively inhibit autoantigen specific CD4+ T cells in a murine arthritis model
Jansen MAA, et al.
Frontiers in Immunology, 10, 2068-2068 (2019)
Sialoside specificity of the siglec family assessed using novel multivalent probes: identification of potent inhibitors of myelin-associated glycoprotein.
Blixt O et al
The Journal of Biological Chemistry, 278(33), 31007-31019 (2003)
Production and characterization of virus-like particles and the P domain protein of GII.4 norovirus.
Koho T et al
Journal of Virological Methods, 179(1), 1-7 (2012)
Development and validation of sandwich quantitative ELISA prototypebased on the bovine IFNg for the detection of cellular immunity
Genc O, et al.
Turkish Journal of Veterinary and Animal Sciences, 39(6), 724-729 (2015)
Ola Blixt et al.
The Journal of biological chemistry, 278(33), 31007-31019 (2003-05-30)
Ten of the 11 known human siglecs or their murine orthologs have been evaluated for their specificity for over 25 synthetic sialosides representing most of the major sequences terminating carbohydrate groups of glycoproteins and glycolipids. Analysis has been performed using
Exibir todos os documentos relacionados

Protocolos

Diethanolamine Assay for the Enzymatic Assay of Alkaline Phosphatase (EC 3.1.3.1)

Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica