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S0902

Sigma-Aldrich

SSC Buffer

for Northern and Southern blotting, powder blend

Sinônimo(s):

Saline-sodium citrate buffer

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About This Item

Código UNSPSC:
41105319
NACRES:
NA.25

Nível de qualidade

200

forma

powder blend

pH

6.8-7.2 (20-25 °C, 263 mg/L in water)

solubilidade

water: 263 mg/mL, clear, colorless

atividade externa

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected

temperatura de armazenamento

room temp

Categorias relacionadas

Descrição geral

SSC Buffer 20x Powder is a standard reagent in Southern and Northern blotting procedures. This concentrated powder should be reconstituted in molecular biology grade water to the indicated final volume before use (final solution is a 20x). The 20x concentrate can be used straight or diluted to make SSC working solutions with concentrations as low as 0.5x.

Aplicação

Saline-sodium citrate (SSC) Buffer has been used as a washing buffer in microarray technique. It has also been used as a constituent of fluorescence in situ hybridization (FISH) buffer.
Suitable for:
  • Preparing nucleic acids for hybridization
  • Hybridization buffer for Northern and Southern blots
  • Washing buffer for Northern and Southern blots

Características e benefícios

  • Multi-purpose use in hybridizations
  • Easily reconstituted and diluted to any concentration needed

Componentes

Final SSC Buffer 20x Concentrate contains 0.3 M sodium citrate in 3M NaCl.

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Código de classe de armazenamento

13 - Non Combustible Solids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

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Bolanos V, et al.
The Plant Journal (2013)
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Papp D, et al.
Autophagy, 12(2), 273-286 (2016)
M Böttger et al.
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Complexes of histones H1 with superhelical SV40 DNA obtained by direct mixing were studied in 0.1 SSC buffer corresponding to 0.02 M Na+. Depending on the molar input ratio H1/DNA three classes of sedimenting species were observed: (1) a component
Aline V Probst et al.
Developmental cell, 19(4), 625-638 (2010-10-19)
At the time of fertilization, the paternal genome lacks the typical configuration and marks characteristic of pericentric heterochromatin. It is thus essential to understand the dynamics of this region during early development, its importance during that time period and how
Christèle Maison et al.
Nature genetics, 43(3), 220-227 (2011-02-15)
HP1 enrichment at pericentric heterochromatin is considered important for centromere function. Although HP1 binding to H3K9me3 can explain its accumulation at pericentric heterochromatin, how it is initially targeted there remains unclear. Here, in mouse cells, we reveal the presence of

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