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Key Documents

H7140

Sigma-Aldrich

Hybridization Solution

for Northern and Southern blotting, liquid (2x concentrate)

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About This Item

Código UNSPSC:
12161700
NACRES:
NA.25

Nível de qualidade

forma

liquid (2x concentrate)

atividade externa

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

temperatura de armazenamento

−20°C

Descrição geral

Sigma′s Hybridization Solution is a 2x ready-to-use hybridization buffer for Northern and Southern blot protocols.

Aplicação

Hybridization Solution has been used to hybridise the nitrocellulose membrane. It has also been used in DNA hybridization.
Suitable for use as a hybridization buffer in Northern and Southern blot protocols.

Componentes

Sigma′s Hybridization Solution contains 200 μg/mL DNA in 10x SSC and 2X Denhardt′s solutions:
200 μg/mL DNA
2mg Bovine Serum Albumin (BSA)
2mg Ficoll
2mg Polyvinylpyrrolidone (PVP)
0.15 M sodium citrate
1.5 M NaCl

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

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