D9905
Denhardt′s Solution
for Northern and Southern blotting, lyophilized powder
Sinônimo(s):
Denhardt′s Solution For Blotting Technique
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About This Item
Código UNSPSC:
12161700
NACRES:
NA.25
Produtos recomendados
grau
for molecular biology
Nível de qualidade
forma
lyophilized powder
técnica(s)
Southern blotting: suitable
atividade externa
DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
temperatura de armazenamento
2-8°C
Descrição geral
Denhardt′s Solution is a blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This powder can be used to make a 50x concentrate that can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.
Aplicação
Denhardt′s Solution has been used:
- as a component in hybridization buffer for the hybridization of brain sections
- in in-situ hybridization peroxidase-(antiperoxidase method-whole mounts) (ISHPAP-WM) process
- in the preparation of hybridization mixture
Suitable as a blocking Buffer for Southern and Northern blot membranes.
Ações bioquímicas/fisiológicas
Denhardt′s solution and gelatin-chrome alum can be used to treat chromosomes for G-banding with trypsin after in situ hybridization.
Componentes
Each vial of Denhardt′s powder contains 50mg Bovine Serum Albumin (BSA), 50mg Ficoll, and 50mg Polyvinylpyrrolidone (PVP).
Nota de preparo
To make a 50x concentrate, dissolve entire contents of vial in 5 mL molecular biology grade water. Store at -20°C for up to two years.
produto relacionado
Nº do produto
Descrição
Preços
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
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