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Key Documents

85639

Sigma-Aldrich

SSC Buffer

for Northern and Southern blotting, powder blend

Sinônimo(s):

Saline sodium citrate buffer dry blend

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About This Item

Código UNSPSC:
12161700
NACRES:
NA.25

grau

for molecular biology

linha de produto

BioReagent

forma

powder blend

Impurezas

DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected

solubilidade

H2O: 0.263 g/mL, clear, colorless

temperatura de armazenamento

room temp

Descrição geral

SSC Buffer powder is a standard reagent in Southern and Northern blotting procedures. This powder can be used to make SSC working solutions with concentrations ranging from 0.5x to 20x.

Aplicação

Suitable for:
  • Preparing nucleic acids for hybridization
  • Hybridization buffer for Northern and Southern blots
  • Washing buffer for Northern and Southern blots

Componentes

  • Convenient pack sizes
  • Multi-purpose use in hybridizations
  • Easy-to-use powder makes fast, inexpensive solutions
SSC Buffer powder contains 0.3 M sodium citrate in 3M NaCl.

Nota de preparo

Dissolve 263.5 g in a final volume of 1L molecular biology grade water. The resulting 20x concentrate is approximately pH 7, and can be further diluted with water, as needed.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)


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