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Documentos Principais

E2636

Sigma-Aldrich

ExtrAvidin® –Alkaline Phosphatase

buffered aqueous solution

Sinônimo(s):

Alkaline Phosphatase Reagent, ExtrAvidin Alkaline Phosphatase

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About This Item

Número MDL:
MFCD00163600
Código UNSPSC:
12352203
NACRES:
NA.46

conjugado

alkaline phosphatase conjugate

Nível de qualidade

200

Formulário

buffered aqueous solution

técnica(s)

dot blot: 1:300,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tissues sections
indirect ELISA: 1:60,000

Condições de expedição

ambient

temperatura de armazenamento

2-8°C

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Descrição geral

Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. It consists of four high affinity binding sites for biotin. ExtrAvidin® is prepared from egg white avidin. It is a modified form of affinity purified avidin with high specific activity of avidin and low background staining of streptavidin. It is a biotin binding protein produced by the bacteria Streptomyces avidinii. ExtrAvidin has been conjugated to Alkaline Phosphatase and is used for various techniques.

Aplicação

ExtrAvidin® -Alkaline Phosphatase has been used in:
  • Dot blot
  • Immunohistochemistry (formalin-fixed, paraffin-embedded sections)
  • Indirect ELISA
  • Enzyme linked immunosorbent assay (ELISA)
  • Enzyme-linked immunosorbent spot (ELISPOT) analysis
  • Competitive ELISA

Ações bioquímicas/fisiológicas

Alkaline Phosphatase catalyzes the hydrolysis of esters to yield phosphoric acid. It is also facilitate the transport of substances across membranes.

forma física

Solution in 0.05 M Tris-HCl buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin and 15 mM sodium azide

Nota de preparo

Affinity purified protein

Informações legais

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

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Certificados de análise (COA)

Lot/Batch Number
088M4782V
21190319
109M4825V
079M4878V
21190318

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Alkaline Phosphatase (2103)
Jenny Meegan et al.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 22(6), 856-862 (2010-11-23)
A competitive enzyme-linked immunosorbent assay (cELISA) was developed by using a whole-cell antigen from a marine Brucella sp. isolated from a harbor seal (Phoca vitulina). The assay was designed to screen sera from multiple marine mammal species for the presence
Joanne L Maki et al.
Clinical and diagnostic laboratory immunology, 10(5), 876-881 (2003-09-11)
Fish acquire protective immunity against the ciliated protozoan parasite Ichthyophthirius multifiliis following sublethal infection or inoculation with I. multifiliis immobilization antigens (i-antigens). In both cases, parasite-immobilizing antibodies have been identified in sera and mucosal secretions. To investigate the kinetics of
Minying Zhang et al.
Nature communications, 9(1), 3919-3919 (2018-09-27)
In addition to genomic mutations, RNA editing is another major mechanism creating sequence variations in proteins by introducing nucleotide changes in mRNA sequences. Deregulated RNA editing contributes to different types of human diseases, including cancers. Here we report that peptides
C E Rutten et al.
Leukemia, 22(7), 1387-1394 (2008-04-18)
Mismatching for human leukocyte antigen (HLA)-DPB1 in unrelated donor hematopoietic stem cell transplantation (URD-SCT) has been associated with a decreased risk of disease relapse, indicating that HLA-DP may represent a target for graft-versus-leukemia (GVL) reactivity in HLA class II-expressing hematological
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