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N2770

Sigma-Aldrich

p-Nitrophenyl phosphate

SIGMAFAST™, chromogenic, tablet

Sinônimo(s):

pNPP tablets

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About This Item

Número CE:
224-246-5
Número MDL:
MFCD00284586
Código UNSPSC:
12352204
NACRES:
NA.83

product name

SIGMAFAST™ p-Nitrophenyl phosphate Tablets, tablet, To prepare 20 mL

Nível de qualidade

200

forma

tablet

solubilidade

water: 0.25 tablet/mL, clear, colorless

temperatura de armazenamento

−20°C

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Aplicação

Developed for use as a soluble substrate for the detection of alkaline phosphatase activity in Enzyme Immunoassays (EIA). pNPP is the substrate of choice in high sensitivity alkaline phosphatase-based EIA assays.One pNPP tablet and one Tris buffer tablet are dissolved in deionized water and provide a ready-to-use substrate. Each Sigma FAST pNPP tablet set yields a solution containing 1.0 mg/ml pNPP, 0.2 M Tris buffer. EIA applications using pNPP may be read in timed assays or stopped with 3N NaOH for delayed readings. SIGMAFAST™ require only the addition of distilled or deionized water to prepare an active substrate solution. Tablets are individually packaged in foil packets.

Reconstituição

Each tablet set dissolved in 20 mL deionized water yields a ready-to-use buffered solution containing pNPP.

Informações legais

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Artigos

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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