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N7653

Sigma-Aldrich

p-Nitrophenyl Phosphate Liquid Substrate System

alkaline phosphatase substrate, liquid

Sinônimo(s):

4-Nitrophenyl phosphate disodium salt solution, pNPP

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About This Item

Fórmula linear:
O2NC6H4OP(O)(ONa)2
Número CAS:
4264-83-9
Peso molecular:
263.05
Número MDL:
MFCD00007319
Código UNSPSC:
12352204
ID de substância PubChem:
24897835
NACRES:
NA.83

product name

p-Nitrophenyl Phosphate Liquid Substrate System, liquid

forma

liquid

Nível de qualidade

200

Condições de expedição

wet ice

temperatura de armazenamento

−20°C

cadeia de caracteres SMILES

[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;/h1-4H,(H2,10,11,12);;/q;2*+1/p-2

chave InChI

VIYFPAMJCJLZKD-UHFFFAOYSA-L

Descrição geral

The p-Nitrophenyl Phosphate (pNPP) Liquid Substrate System combines p-nitrophenyl phosphate, buffer, and the required magnesium cations in a convenient, ready-to-use, single solution reagent. Producing a soluble end product, pNPP is the substrate of choice in alkaline phosphatase enzyme immunoassays.

Aplicação

p-Nitrophenyl Phosphate Liquid Substrate System has been used as a substrate to determine the activity of alkaline phosphatase in alkaline phosphatase assay.
Substrate for alkaline phosphatase that yields a soluble yellow end product that may be read at 405 nm. The reaction may be stopped with 3N NaOH and read at 405 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.

Ações bioquímicas/fisiológicas

4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP produces p-nitrophenol. It is a common chromogenic substrate for alkaline phosphatase.

Atenção

Stable at least one year when stored below 0 °C.

forma física

Ready-to-use.

Pictogramas

Health hazardCorrosion
GHS08,GHS05

Palavra indicadora

Danger

Classificações de perigo

Eye Dam. 1 - Met. Corr. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral

Órgãos-alvo

Kidney,Liver,Blood

Código de classe de armazenamento

8B - Non-combustible corrosive hazardous materials

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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European journal of medicinal chemistry, 178, 108-115 (2019-06-09)
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Artigos

High-throughput Screening of Peptide Substrates for Tyrosine Kinases Using PEPscreen® Custom Peptide Library

Review a high-throughput ELISA-based method to identify peptide substrates for class-specific and/or enzyme-specific Protein Tyrosine Kinases (PTKs) that can be utilized for the detection of kinase activity both in vivo and in vitro.

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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