10567752001

Alkaline Phosphatase

EIA grade, from calf intestine

• Sinônimo(s): AP, Alkaline Phosphatase
• Número da licença da enzima: 3.1.3.1 (BRENDA, IUBMB)
• Código UNSPSC: 12352204

Propriedades

• fonte biológica: bovine (calf) intestine
• Nível de qualidade: 100
• forma: solution
• atividade específica: ~3,000 U/mg enzyme protein (+37°C, 4-nitrophenyl phosphate substrate, diethanolamine as buffer)
• embalagem: pkg of 1.5 mL (15 mg)
• fabricante/nome comercial: Roche
• pH ideal: 9.8
• faixa de pH: 9.0-11.0
• Condições de expedição: wet ice

Descrição

Descrição geral

Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
Amino groups: 8-16 moles/mole enzyme

Aplicação

Alkaline phosphatase can be coupled to other proteins via its amino groups. The enzyme solution can be used directly for conjugation without prior dialysis.

Qualidade

Purity: single peak (HPLC)

Sequência

Purification procedure
AP from intestine is a dimeric glycoprotein, it is not known, whether the carbohydrate part is separated during purification procedure. Content of amino groupsIn the literature the carbohydrate content is 2 to 20 percent so one should find approx. 70 amino groups/mol.
In addition one researcher determined the amino groups and found 50 to 60 lysins/mol.-SH groups in AP
No information in literature about -SH groups in AP.

Carbohydrate structure
Results of investigation of the carbohydrate structure of several lots of alkaline phosphatase are available.

NH₂- and carbohydrate estimation
Results of NH₂- and carbohydrate estimation are available.

Definição da unidade

Unit Conversion: Approx. 4.35 U (EIA grade AP) [37 °C, 4-NPP as substrate, diethanolamine as buffer, pH 9.8] = 1 U [+25 °C, 4-NPP as substrate, glycine as buffer, pH 10.5]
1.0 U [+37 °C, QC assay conditions] = 0.73 U [+30 °C, QC assay conditions] = 0.48 U [+25 °C, QC assay conditions]

forma física

Solution, ready-to-use, 10mg/ml

Nota de preparo

Storage conditions (working solution): The diluted enzyme (1:10 in 3 M NaCl) will give a stability of 6 months at 4 °C.
1:10 dilution in water will give stability for 1 week at 4°C.
Do not freeze the diluted or undiluted enzyme, because freezing will destroy enzyme activity.

Armazenamento e estabilidade

Store at 2 to 8 °C. (Do not freeze)

Outras notas

For life science research only. Not for use in diagnostic procedures.

Informações de segurança

• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): does not flash
• Ponto de fulgor (°C): does not flash