Pular para o conteúdo
Merck
Todas as fotos(1)

Key Documents

View All Documentation

79390

Sigma-Aldrich

Phosphatase, Alkaline from calf intestinal mucosa

suitable for enzyme immunoassay, solution (clear, colorless), ~2500 U/mg protein (~10 mg/ml)

Sinônimo(s):

Alkaline Phosphatase from calf intestinal mucosa

Faça loginpara ver os preços organizacionais e de contrato
Todas as fotos(1)

About This Item

Número CAS:
9001-78-9
Número da licença da enzima:
3.1.3.1 (BRENDA, IUBMB)
Número CE:
232-631-4
Número MDL:
MFCD00131849
Código UNSPSC:
12352204
eCl@ss:
42010105
NACRES:
NA.54

fonte biológica

bovine (calf) intestinal mucosa

forma

solution (clear, colorless)

atividade específica

~2500 U/mg protein (~10 mg/ml)

peso molecular

Mr ~140000

técnica(s)

enzyme immunoassay: suitable

Impurezas

0.1 mM ZnCl2
1 mM MgCl2
3 M NaCl
30 mM triethanolamine

pH

~7.6

temperatura de armazenamento

2-8°C

Procurando produtos similares? Visita Guia de comparação de produtos

Aplicação

Alkaline phosphatase is used in the laboratory to remove phosphate monoesters which prevents self-ligation. It is also used in the dairy industry to confirm pasturization of milk. Alkaline phosphatse is commonly conjugated to antibodies and used in immunoblotting, ELISA′s, and other antibody based detection assays.

Ações bioquímicas/fisiológicas

Alkaline phosphatase is a hydrolytic enzyme which dephosphorylates a variety of molecules under basic conditions.

Definição da unidade

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol 4-nitrophenyl phosphate per minute at pH 9.8 and 37 °C

Reconstituição

The solution can be used without dialysis (for enzyme immuno-assay).

Outras notas

Sales restrictions may apply
Used in highly sensitive (pg/m) enzyme immunoassays with LCEC detection of phenol; Comparison of three methods for conjugating alkaline phosphatase to human placental lactogen; Preparation of enzyme-antibody conjugates; Liposome immunoassay with antigen-coupled liposomes containing alkaline phosphatase

Pictogramas

Health hazard
GHS08

Palavra indicadora

Danger

Frases de perigo

H334

Declarações de precaução

P261 - P284 - P501

Classificações de perigo

Resp. Sens. 1

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

M.I. Halliday et al.
Biochemical Society Transactions, 14, 473-473 (1986)
K.R. Wehrmeyer et al.
Journal of Liquid Chromatography, 6, 2141-2141 (1983)
D G Williams
Journal of immunological methods, 72(1), 261-268 (1984-08-03)
One of the requirements for enzyme immunoassay is the formation of a labelled component of the assay system which can be either the ligand or the binder. Three methods for conjugating alkaline phosphatase to human placental lactogen were investigated, involving
Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes.
E Engvall et al.
Journal of immunology (Baltimore, Md. : 1950), 109(1), 129-135 (1972-07-01)
C K Kim et al.
Journal of immunological methods, 159(1-2), 101-106 (1993-02-26)
Immunoliposomes were prepared and their immunoassay applications investigated. Liposomes were prepared from cholesterol and phospholipids including maleimidobenzoylphosphatidylethanolamine (MBPE) for conjunction with thiol-containing antigens. Alkaline phosphatase (APase) was entrapped in the liposome and BSA, the antigen, was modified by reaction with
Exibir todos os documentos relacionados

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica