T6066

Sigma-Aldrich

Trizma® base

BioPerformance Certified, meets EP, USP testing specifications, suitable for cell culture, ≥99.9% (titration)

Synonym(s):
Tris base, Trometamol, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris(hydroxymethyl)aminomethane
Linear Formula:
NH2C(CH2OH)3
CAS Number:
Molecular Weight:
121.14
Beilstein/REAXYS Number:
741883
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

grade

BioPerformance Certified

Quality Level

Agency/Method

USP/NF
meets EP testing specifications
meets USP testing specifications

description

aminopeptidase substrate

assay

≥99.9% (titration)

form

crystalline powder

application(s)

cell culture | mammalian: suitable

impurities

DNase, RNase, protease, none detected
Endotoxin and Total Count Aerobic, Yeast, plus Mold, tested
≤0.2% water (Karl Fischer)

loss

≤0.5% (loss on drying, 110 °C)

pH

10.0-11.5

useful pH range

7 - 9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)

solubility

water: soluble (666 mg/ml, yielding a clear colorless solution)

absorption

≤0.05 at 260 at 1 M
≤0.05 at 290 at 40%
≤0.06 at 280 at 1 M

Featured Industry

Diagnostic Assay Manufacturing

SMILES string

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

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General description

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Application

Trizma has been used:
  • As a component in the culture of human embryonic stem (hES) cells on human and mouse feeder cells
  • As a component of pre-hybridization buffer in in situ hybridization
  • To suspend dried protein before SDS-PAGE protein characterization
  • As a component of lysis buffer to wash and lyse cells before immunoprecipitation
  • In the extraction and enrichment of membrane proteins for separation by two-dimensional polyacrylamide gel electrophoresis
  • In studies of double stranded complexes of peptide nucleic acids (PNA) and their complementary DNA sequences, by use of anion exchange HPLC
  • In capillary electrochromatography and UV analysis of tocopherols and tocotrienols

Packaging

1 kg in poly bottle
100, 500 g in poly bottle
5 kg in poly drum
T6066-1KT:
Each kit contains 3 x 100G samples, each sample from a uniquely manufactured lot.

Other Notes

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Easily compare specifications for Trizma products with the Trizma specification table.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

storage_class_code

11 - Combustible Solids

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Culture of human embryonic stem cells on human and mouse feeder cells.
Turksen, Kursad
Human Embryonic Stem Cell Protocols, 91-104 (2006)
Recurrent mutations in the MTOR regulator RRAGC in follicular lymphoma.
Peterson LF, et al.
Clinical Cancer Research, 22(21), 5383-5393 (2016)
Membrane proteins and proteomics: un amour impossible?
Santoni V, et al.
Electrophoresis, 21(6), 1054-1070 (2000)
Recognition and strand displacement of DNA oligonucleotides by peptide nucleic acids (PNAs). High-performance ionexchange chromatographic analysis
Lesignoli F, et al.
Journal of Chromatography A, 922(1-2), 177-185 (2001)
Capillary electrochromatographic evaluation of vitamin active oil constituents: tocopherols and tocotrienols.
Abidi SL and Rennick KA
Journal of Chromatography A, 913(1-2), 379-386 (2001)
Articles
The Comet Assay, also called single cell gel electrophoresis (SCGE), is a sensitive and rapid technique for quantifying and analyzing DNA damage in individual cells.
Read More
Protocols
Creating Transgenic Mice using CRISPR-Cas9 Genome Editing
Read More

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