Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.
Tris base has been used:
- To prepare Tris-HCl, which is used as a component of lysis buffer for protein characterization
- In the preparation of Tris-buffered saline Tween-20 (TBST) buffer for Western blotting
- In the preparation of TBS (Tris-buffered saline) that is used for dialysis to neutralize a protein
- As a component of nuclei extraction buffer
2.5 kg in poly drum
1 kg in poly bottle
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
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