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RDD008

Sigma-Aldrich

Trizma® base

anhydrous, free-flowing, Redi-Dri, ≥99.9%

Synonym(s):
2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
Linear Formula:
NH2C(CH2OH)3
CAS Number:
Molecular Weight:
121.14
Beilstein:
741883
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

grade

anhydrous

Quality Level

description

aminopeptidase substrate

product line

Redi-Dri

assay

≥99.9%

form

powder

quality

free-flowing

impurities

≤0.2% Water (Karl Fischer)

color

white

pH

10.5-12
7-9

useful pH range

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)

solubility

water: soluble (Water < 0.2%)

absorption

≤0.05 at 290 nm at 40%

λ

(A290 UV absorbance < 0.05)
in 5 mM Tris; DNA (40% (w/w))

SMILES string

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

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General description

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology.[00] It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc.[01] It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.[49]

Application

Tris base has been used:
  • To prepare Tris-HCl, which is used as a component of lysis buffer for protein characterization
[45]
  • In the preparation of Tris-buffered saline Tween-20 (TBST) buffer for Western blotting
  • [46]
  • In the preparation of TBS (Tris-buffered saline) that is used for dialysis to neutralize a protein
  • [47]
  • As a component of nuclei extraction buffer
  • [48]

    Packaging

    1 kg in poly bottle
    2.5 kg in poly drum

    Other Notes

    The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
    For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

    Legal Information

    Redi-Dri is a trademark of Sigma-Aldrich Co. LLC
    Trizma is a registered trademark of Sigma-Aldrich Co. LLC

    Storage Class Code

    11 - Combustible Solids

    WGK

    WGK 1

    Flash Point(F)

    Not applicable

    Flash Point(C)

    Not applicable

    Certificate of Analysis

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    Certificate of Origin

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