T4661
Trizma® base
≥99.9% (titration), crystalline
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description
aminopeptidase substrate
Quality Level
Assay
≥99.9% (titration)
form
crystalline
loss
≤5% (loss on drying, 110 °C)
color
white
pH
10.0-11.5
useful pH range
7-9
pKa (25 °C)
8.1
bp
219-220 °C/10 mmHg (lit.)
mp
167-172 °C (lit.)
solubility
water: soluble (400 g plus 600 mL of water)
λ
(290nm < 0.05)
in 5 mM Tris; DNA (40% (w/w))
application(s)
diagnostic assay manufacturing
SMILES string
NC(CO)(CO)CO
InChI
1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
InChI key
LENZDBCJOHFCAS-UHFFFAOYSA-N
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This Item | T6791 | T6066 | T1503 |
|---|---|---|---|
| assay ≥99.9% (titration) | assay ≥99.9% (titration) | assay ≥99.9% (titration) | assay ≥99.9% (titration) |
| form crystalline | form crystalline | form crystalline powder | form crystalline |
| color white | color - | color - | color - |
| pH 10.0-11.5 | pH 10.5-12.0 (1 M in H2O) | pH 10.0-11.5 | pH 10.5-12 |
| mp 167-172 °C (lit.) | mp 167-172 °C (lit.) | mp 167-172 °C (lit.) | mp 167-172 °C (lit.) |
| solubility water: soluble (400 g plus 600 mL of water) | solubility H2O: 1 M, colorless | solubility water: soluble (666 mg/ml, yielding a clear colorless solution) | solubility methanol: soluble 26 mg/mL at 25 °C, ethylene glycol: soluble 79.1 mg/mL at 25 °C, water: soluble (678 g/l at 20 °C) |
General description
Application
- As a component of lysis buffer for cell disruption
- In the preparation of TBE solution for PAGE (polyacrylamide gel electrophoresis)
- In studies of double stranded complexes of peptide nucleic acids (PNA) and their complementary DNA sequences, by use of anion exchange HPLC
- In capillary electrochromatography and UV analysis of tocopherols and tocotrienols
Packaging
Analysis Note
Other Notes
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
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