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Trizma® base

BioUltra, for molecular biology, ≥99.8% (T)

Tris base, Trometamol, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris(hydroxymethyl)aminomethane
Linear Formula:
CAS Number:
Molecular Weight:
EC Number:
MDL number:
PubChem Substance ID:

Quality Level


for molecular biology


aminopeptidase substrate

product line



≥99.8% (T)




DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

ign. residue

≤0.01% (as SO4)


≤0.5% loss on drying, 110 °C


colorless to white


10.5-12.0 (25 °C, 4 M in H2O)

useful pH range


pKa (25 °C)



219-220 °C/10 mmHg (lit.)


167-172 °C (lit.)
168-172 °C


H2O: 4 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤20 mg/kg
sulfate (SO42-): ≤5 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

SMILES string



4 M in H2O

UV absorption

λ: 260 nm Amax: 0.10
λ: 280 nm Amax: 0.08


in accordance for luminescence



InChI key


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General description

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

The BioChemika quality is for the usual biochemical applications. The products designated as BioChemika Ultra grade are suitable for different applications like purification, precipitation, crystallization and other applications which require tight control of elemental content. Trace elemental analyses have been performed for all qualities. The molecular biology qualities are also tested for absence of nucleases and the luminescence quality spectroscopic tests are performed.


Trizma has been used:
  • In the dissection medium and culture medium of Hippocampal organotypic tissue cultures
  • As a component of lysis buffer before immunoprecipitation and immunoblotting
  • As a component of harvest buffer, lysis buffer, ChIP dilution buffer, and wash buffer in chromatin immunoprecipitation method for studying protein-DNA binding
  • As a component of mixed buffer formulations
  • As a component of NuPAGE loading buffer for SDS-PAGE and as a component of transfer buffer for Western blotting
Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Trizma based buffers are also in column chromatography and in gel electrophoresis. Trizma base is used as a general reagent for the preparation of all types of Tris buffers.

Other Notes

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Easily compare specifications for Trizma products with the Trizma specification table.
Running buffer component in non-denaturing agarose gel electrophoresis; In non-denaturing and urea-denaturing polyacrylamide gel electrophoresis; DNA digest analysis with capillary electrophoresis.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

R.C. Ogden et al.
Methods in Enzymology, 152, 78-78 (1987)
A new method allowing long-term potentiation recordings in hippocampal organotypic slices.
Paci P, et al.
Brain and Behavior, 7(5), e00692-e00692 (2017)
In vitro Detection of S-acylation on Recombinant Proteins via the Biotin-Switch Technique.
Qi D and Innes RW
Bio-protocol, 4(22) (2014)
A fast, efficient chromatin immunoprecipitation method for studying protein-DNA binding in Arabidopsis mesophyll protoplasts.
Plant methods, 13(1), 42-42 (2017)
G G Nahas et al.
Drugs, 55(2), 191-224 (1998-03-20)
THAM (trometamol; tris-hydroxymethyl aminomethane) is a biologically inert amino alcohol of low toxicity, which buffers carbon dioxide and acids in vitro and in vivo. At 37 degrees C, the pK (the pH at which the weak conjugate acid or base


Restriction Enzyme Cloning Manual Buffer Recipes

TE Buffer; Elution Buffer; 10x Ligation Buffer; 0.5 M PIPES Buffer; Inoue Transformation Buffer

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