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T3253

Sigma-Aldrich

Trizma® hydrochloride

reagent grade, ≥99.0% (titration), crystalline

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Synonym(s):
TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride
Linear Formula:
NH2C(CH2OH)3 · HCl
CAS Number:
1185-53-1
Molecular Weight:
157.60
Beilstein:
3675235
EC Number:
214-684-5
MDL number:
MFCD00012590
PubChem Substance ID:
24900119
NACRES:
NA.25

grade

reagent grade

Assay

≥99.0% (titration)

form

crystalline

impurities

≤0.5% water (Karl Fischer)

useful pH range

7.0-9.0

pKa (25 °C)

8.1

mp

150-152 °C

solubility

H2O: soluble, clear, colorless

absorption

≤0.05 at 290 nm at 40%

application(s)

diagnostic assay manufacturing

SMILES string

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

InChI key

QKNYBSVHEMOAJP-UHFFFAOYSA-N

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This Item
T5941T15760T6666
Trizma® hydrochloride reagent grade, ≥99.0% (titration), crystalline

Sigma-Aldrich

T3253

Trizma® hydrochloride

Trizma® hydrochloride BioPerformance Certified, suitable for cell culture, ≥99.0% (titration)

Sigma-Aldrich

T5941

Trizma® hydrochloride

Trizma® hydrochloride reagent grade, ≥99%, crystalline

Sigma-Aldrich

T15760

Trizma® hydrochloride

Trizma® hydrochloride pH 3.5-5.0 (0.5 M in H2O), BioXtra, ≥99.0% (titration)

Sigma-Aldrich

T6666

Trizma® hydrochloride

assay

≥99.0% (titration)

assay

≥99.0% (titration)

assay

≥99%

assay

≥99.0% (titration)

form

crystalline

form

crystalline powder

form

crystalline

form

powder

impurities

≤0.5% water (Karl Fischer)

impurities

DNAse, Exonuclease; NICKase, Endonuclease; RNAse and Protease, none detected, endotoxin and total aerobic microbial count, tested, ≤0.5% water (Karl Fischer)

impurities

-

impurities

Insoluble matter, passes filter test

mp

150-152 °C

mp

150-152 °C

mp

-

mp

150-152 °C

solubility

H2O: soluble, clear, colorless

solubility

H2O: 667 mg/mL

solubility

-

solubility

H2O: 0.5 M, clear, colorless

General description

Trizma® hydrochloride is mainly required for preparation of buffer at physiological range of 7.3 to 7.5. The prepared buffer is compatible with biological fluids. It is of importance to laboratories as a standard pH solution.

Application

Trizma® hydrochloride has been used as a buffer solution for staining procedure, reduction and alkylation studies and in situ hybridization procedure.­

Other Notes

Easily compare specifications for Trizma HCl products with the Trizma HCl specification table.
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

also commonly purchased with this product

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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25G
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Trizma® base ≥99.9% (titration), crystalline

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Tris, Hydrochloride, Molecular Biology Grade Useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C.

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Tris base =99.9% (titration), abs., 100 mg/mL, 300 nm ≤0.015

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