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T6666

Sigma-Aldrich

Trizma® hydrochloride

pH 3.5-5.0 (0.5 M in H2O), BioXtra, ≥99.0% (titration)

Synonym(s):
TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride
Linear Formula:
NH2C(CH2OH)3 · HCl
CAS Number:
Molecular Weight:
157.60
Beilstein:
3675235
EC Number:
MDL number:
PubChem Substance ID:

product line

BioXtra

Quality Level

Assay

≥99.0% (titration)

form

powder

impurities

Insoluble matter, passes filter test

ign. residue (900 °C)

≤0.2% (as SO4)

loss

≤0.2% loss on drying, 110°C

pH

3.5-5.0 (0.5 M in H2O)

useful pH range

7.0-9.0

pKa (25 °C)

8.1

mp

150-152 °C

solubility

H2O: 0.5 M, clear, colorless

anion traces

sulfate (SO42-): ≤0.005%

cation traces

Al: ≤0.0005%
As: ≤0.0001%
Ba: ≤0.0005%
Bi: ≤0.0005%
Ca: ≤0.001%
Cd: ≤0.0005%
Co: ≤0.0005%
Cr: ≤0.0005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Li: ≤0.0005%
Mg: ≤0.0005%
Mn: ≤0.0005%
Mo: ≤0.0005%
Na: ≤0.005%
Ni: ≤0.0005%
Pb: ≤0.0005%
Sr: ≤0.0005%
Zn: ≤0.0005%

absorption

≤0.010 at 280 in H2O at 0.5 M
≤0.015 at 260 in H2O at 0.5 M

application(s)

diagnostic assay manufacturing

SMILES string

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

InChI key

QKNYBSVHEMOAJP-UHFFFAOYSA-N

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This Item
T3253T5941T15760
Trizma® hydrochloride pH 3.5-5.0 (0.5 M in H2O), BioXtra, ≥99.0% (titration)

Sigma-Aldrich

T6666

Trizma® hydrochloride

Trizma® hydrochloride reagent grade, ≥99.0% (titration), crystalline

Sigma-Aldrich

T3253

Trizma® hydrochloride

Trizma® hydrochloride BioPerformance Certified, suitable for cell culture, ≥99.0% (titration)

Sigma-Aldrich

T5941

Trizma® hydrochloride

product line

BioXtra

product line

-

product line

-

product line

-

assay

≥99.0% (titration)

assay

≥99.0% (titration)

assay

≥99.0% (titration)

assay

≥99%

form

powder

form

crystalline

form

crystalline powder

form

crystalline

impurities

Insoluble matter, passes filter test

impurities

≤0.5% water (Karl Fischer)

impurities

DNAse, Exonuclease; NICKase, Endonuclease; RNAse and Protease, none detected, endotoxin and total aerobic microbial count, tested, ≤0.5% water (Karl Fischer)

impurities

-

pH

3.5-5.0 (0.5 M in H2O)

pH

-

pH

-

pH

-

mp

150-152 °C

mp

150-152 °C

mp

150-152 °C

mp

-

Application

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Other Notes

Easily compare specifications for Trizma HCl products with the Trizma HCl specification table.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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