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10812846001

Roche

Tris hydrochloride

>99% (titration), pH 7.0-9.0, suitable for FISH

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Synonym(s):
Trizma® hydrochloride, TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride
Linear Formula:
NH2C(CH2OH)3 · HCl
CAS Number:
1185-53-1
Molecular Weight:
157.60
Beilstein:
3675235
MDL number:
MFCD00012590
PubChem Substance ID:
329749168

Assay

>99% (titration)

form

solid

packaging

pkg of 500 g

manufacturer/tradename

Roche

technique(s)

FISH: suitable

pH

7.0-9.0

useful pH range

7.0-9.0

pKa (25 °C)

8.1

absorption

<0.02 at 300 nm at 100 mg/mL

storage temp.

room temp

SMILES string

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

InChI key

QKNYBSVHEMOAJP-UHFFFAOYSA-N

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This Item
93363T3253648317
Tris hydrochloride &gt;99% (titration), pH 7.0-9.0, suitable for FISH

Roche

10812846001

Tris hydrochloride

Trizma&#174; hydrochloride BioUltra, for molecular biology, &#8805;99.0% (AT)

Sigma-Aldrich

93363

Trizma® hydrochloride

Trizma&#174; hydrochloride reagent grade, &#8805;99.0% (titration), crystalline

Sigma-Aldrich

T3253

Trizma® hydrochloride

Tris, Hydrochloride, Molecular Biology Grade Useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25&#176;C.

Millipore

648317

Tris, Hydrochloride, Molecular Biology Grade

form

solid

form

powder or crystals

form

crystalline

form

crystalline solid

technique(s)

FISH: suitable

technique(s)

-

technique(s)

-

technique(s)

-

pH

7.0-9.0

pH

2.5-4.0 (25 °C, 4 M in H2O)

pH

-

pH

-

storage temp.

room temp

storage temp.

-

storage temp.

-

storage temp.

15-25°C

packaging

pkg of 500 g

packaging

-

packaging

-

packaging

-

Application

Buffering agent in incubation mixtures. It has also been used as a component of lysis and TE (Tris-EDTA) buffer.

Features and Benefits

Crystals

Quality

Purity: >99% Tris-HCl (titrimetric)
Heavy metals: <1 ppm Pb and Fe

Other Notes

For life science research only. Not for use in diagnostic procedures.
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Protein lysine methylation mediates a variety of biological processes, and their dysregulation has been established to play pivotal roles in human disease. A number of these sites constitute attractive drug targets. However, systematic identification of methylation sites is challenging and
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