cell culture | mammalian: suitable
DNAse, Exonuclease; NICKase, Endonuclease; RNAse and Protease, none detected
endotoxin and total aerobic microbial count, tested
≤0.5% water (Karl Fischer)
H2O: 667 mg/mL
≤0.05 at 290 at 40%
suitable for electrophoresis
diagnostic assay manufacturing
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powder or crystals
cell culture | mammalian: suitable, electrophoresis: suitable
DNAse, Exonuclease; NICKase, Endonuclease; RNAse and Protease, none detected, endotoxin and total aerobic microbial count, tested, ≤0.5% water (Karl Fischer)
Insoluble matter, passes filter test
≤0.5% water (Karl Fischer)
DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected
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Selection of Poly(A)+ RNA by Oligo(dT)-Cellulose Chromatography
Membrane-based blotting applications that employ enzyme conjugates to generate colorimetric or chemiluminescent signal require the use of an added blocking step to decrease the signal generated by non-specific binding.
In Situ Hybridization of Whole-Mount Mouse Embryos with RNA Probes: Hybridization, Washes, and Histochemistry. This is a protocol describing how to perform in situ hybridization on whole mouse embryos. Here we describe the hybridization procedure, and the localization of the DIG-labeled RNA using a conjugate of anti-DIG Fab antibody and calf intestinal alkaline phosphatase. Enzyme activity of the reporter is detected by a color reaction, resulting in the formation of a water-insoluble purple/blue precipitate. Manipulating the Mouse Embryo - Third Edition