252859

Sigma-Aldrich

Tris(hydroxymethyl)aminomethane

ACS reagent, ≥99.8%

Synonym(s):
Tris base, Trometamol, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM
Linear Formula:
NH2C(CH2OH)3
CAS Number:
Molecular Weight:
121.14
Beilstein/REAXYS Number:
741883
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

Quality Level

grade

ACS reagent

assay

≥99.8%
99.8-100.1%

form

crystalline

impurities

≤0.005% insolubles
≤2% water

useful pH range

7 - 9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)

cation traces

Fe: ≤5 ppm
heavy metals (as Pb): ≤5 ppm

absorption

passes test

SMILES string

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

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General description

Tris(hydroxymethyl)aminomethane is mainly required for preparation of buffers at physiological range of 7.3 to 7.5. The prepared buffers are compatible with biological fluids. It is of importance to laboratories as a standard pH solution.

Application

Tris(hydroxymethyl)aminomethane has been used as a buffer solution for lactate dehydrogenase assay, in situ hybridization procedure and protein extraction from cells.

Packaging

100, 500 g in poly bottle

Other Notes

Easily compare specifications for Trizma products with the Trizma specification table.

storage_class_code

11 - Combustible Solids

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

An optimized lactate dehydrogenase release assay for screening of drug candidates in neuroscience.
Kaja S et al.
Journal of Pharmacological and Toxicological Methods, 73, 1-1 (2015)
Fernando E Díaz-Manzano et al.
Frontiers in plant science, 7, 966-966 (2016-07-28)
Galls induced by Meloidogyne spp. in plant roots are a complex organ formed by heterogeneous tissues; within them there are 5-8 giant cells (GCs) that root-knot nematodes use for their own nurturing. Subtle regulatory mechanisms likely mediate the massive gene...
Tris-tris-HCl: a standard buffer for use in the physiologic pH range.
R A Durst et al.
Clinical chemistry, 18(3), 206-208 (1972-03-01)
Jianbin Zhang et al.
Autophagy, 10(5), 901-912 (2014-03-29)
Autophagy is a catabolic process during which cellular components including protein aggregates and organelles are degraded via a lysosome-dependent process to sustain metabolic homeostasis during nutrient or energy deprivation. Measuring the rate of proteolysis of long-lived proteins is a classical...
Thierry D G A Mondeel et al.
Nucleic acids research, 47(15), 7825-7841 (2019-07-13)
The understanding of the multi-scale nature of molecular networks represents a major challenge. For example, regulation of a timely cell cycle must be coordinated with growth, during which changes in metabolism occur, and integrate information from the extracellular environment, e.g....

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