252859
Tris Base
99.8-100.1%,≥99.8%, crystalline, ACS reagent
Synonym(s):
Tris(hydroxymethyl)aminomethane, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Trometamol
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All Photos(3)
About This Item
Linear Formula:
NH2C(CH2OH)3
CAS Number:
Molecular Weight:
121.14
Beilstein/REAXYS Number:
741883
EC Number:
MDL number:
UNSPSC Code:
12352104
PubChem Substance ID:
NACRES:
NA.25
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product name
Tris(hydroxymethyl)aminomethane, ACS reagent, ≥99.8%
grade
ACS reagent
Quality Level
assay
≥99.8%
99.8-100.1%
form
crystalline
technique(s)
affinity chromatography: suitable
impurities
≤0.005% insolubles
≤2% water
color
white
useful pH range
7-9
pKa (25 °C)
8.1
bp
219-220 °C/10 mmHg (lit.)
mp
167-172 °C (lit.)
cation traces
Fe: ≤5 ppm
heavy metals: ≤5 ppm (ICP)
absorption
passes test
application(s)
clinical research
diagnostic assay manufacturing
microbiology
foreign activity
DNase, none detected
NICKase, none detected
RNase, none detected
protease, none detected
SMILES string
NC(CO)(CO)CO
InChI
1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
InChI key
LENZDBCJOHFCAS-UHFFFAOYSA-N
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General description
Tris(hydroxymethyl)aminomethane, commonly known as Trometamol, Tris base, or Trizma base, plays a crucial role in diverse research applications as a biological buffer. It is extensively used in the formulation of buffers like Tris-acetate-EDTA (TAE) and Tris-borate-EDTA (TBE), owing to its optimal pKa of 8.1, making it well-suited for maintaining pH within the physiological range (pH 7 -9) applicable to most living organisms. However, caution might be necessary when studying some proteins, as Tris has the potential to interfere with the activity of certain enzymes. Tris base can be employed both independently as a buffer and as a component in mixed buffer formulations, including Tris-EDTA (TE) buffer, TAE buffer, and TBE buffer, among others. Importantly, Tris base is characterized by its purity, essential stability, and relative non-hygroscopic nature.
In laboratory settings, Tris base is indispensable for the preparation of buffers compatible with biological fluids, acting as a standard pH solution. It facilitates various procedures such as lactate dehydrogenase assays, in situ hybridization, and protein extraction from cells. Its versatility extends to applications in cell culture, biochemistry, and molecular biology labs, where it contributes to studies involving cell membrane permeability and buffer preparation.
In laboratory settings, Tris base is indispensable for the preparation of buffers compatible with biological fluids, acting as a standard pH solution. It facilitates various procedures such as lactate dehydrogenase assays, in situ hybridization, and protein extraction from cells. Its versatility extends to applications in cell culture, biochemistry, and molecular biology labs, where it contributes to studies involving cell membrane permeability and buffer preparation.
Application
Tris(hydroxymethyl)aminomethane has been used:
- as a buffer solution for lactate dehydrogenase assay, in situ hybridization procedure and protein extraction from cells.
- as a buffer in flow cell sample preparation for Raman spectroscopy
- as a wash buffer in Western blotting
- as a buffer component in decellularization of tissues
Features and Benefits
- Efficient buffering within the pH range of 7-9 with a pKa of 8.1 (25 °C)
- Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
- Can be used in Cell Biology, and Biochemical research
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Legal Information
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
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