Triton X-100

laboratory grade

t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether
Linear Formula:
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
CAS Number:
Beilstein/REAXYS Number:
MDL number:
PubChem Substance ID:

Quality Level


laboratory grade





mol wt

micellar avg mol wt 80,000
average mol wt 625

aggregation number



0.2-0.9 mM (20-25°C)

transition temp

cloud point 65 °C
pour point ~7 °C


water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow



SMILES string




InChI key


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General description

Triton X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.
Triton X-100 has hemolytic property and is used for DNA extraction.


Triton-X has been used:
  • In the permeabilization of cells for immunofluorescence staining
  • As a component of lysis buffer in western blot analysis
  • As a component of Tris-buffered saline for the preparation of cell sections in Immunogold labelling for electron microscope


5 mL in glass bottle
1 gal in poly bottle
5 gal in poly drum
1 L in poly bottle
100, 500 mL in poly bottle

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Legal Information

Tri-Chek is a trademark of Micro Essential Laboratory, Inc.
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Signal Word


Hazard Statements

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


UN 3082 9 / PGIII

WGK Germany


Flash Point(F)

483.8 °F

Flash Point(C)

251 °C

Certificate of Analysis

Certificate of Origin

Growth and hydration of Triton X-100 micelles in monovalent alkali salts: a light scattering study
Molina-Bolivar JA, et al.
The Journal of Physical Chemistry B, 106(4), 870-877 (2002)
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