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93422

Sigma-Aldrich

Triton X-114

Synonym(s):
(1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol, Polyethylene glycol tert-octylphenyl ether
Linear Formula:
(C2H4O)n C14H22O, n = 7 or 8
CAS Number:
Beilstein:
2315025
MDL number:
PubChem Substance ID:
NACRES:
NA.21

Quality Level

description

non-ionic

mol wt

average mol wt 537

impurities

≤0.001% peroxides (as H2O2)
≤1% water

ign. residue

≤0.2%

refractive index

n20/D 1.488 (lit.)
n20/D 1.495

CMC

0.2 mM (20-25°C)

transition temp

pour point ~−9 °C
cloud point 23 °C

density

1.052 g/mL at 20 °C
1.058 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤50 mg/kg

cation traces

Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤15 mg/kg
K: ≤500 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Na: ≤500 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Zn: ≤5 mg/kg

HLB

12.4

SMILES string

CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

InChI

1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3

InChI key

HNLXNOZHXNSSPN-UHFFFAOYSA-N

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General description

Triton X-114 is a nonionic detergent. It is a good detergent for solubilizing membranes having reversible phase-separation property useful for the separation of intrinsic membrane proteins. Solution of Triton X-114 is homogeneous at 0°C but phase separation into aqueous phase and a detergent phase occurs above 20°C. Phase separation of proteins in solutions of Triton X-114 has been investigated. This octylphenol ethoxylate is compatible with anionic, cationic, and other nonionic surfactants and chemically stable in most acidic & alkaline solutions.

Application

Triton X-114 has been used:
  • In phase separation of integral membrane proteins
  • In the purification of bacterial inclusion bodies
  • To study the clouding phenomenon in promethazine hydrochloride, an amphiphilic phenothiazine drug
  • To partition conditioned media into insoluble and soluble phases before Western blotting
  • For solubilization of phosphatidylinositol 4-phosphate 5-kinase from bovine brain

Packaging

1 L in glass bottle
250 mL in glass bottle

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

483.8 °F

Flash Point(C)

251 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Certificate of Origin

More documents

Quotes and Ordering

Cloud point variation of amphiphilic drug promethazine hydrochloride with added surfactants.
Naqvi A Z and Khatoon S
Colloids and Surfaces. B, Biointerfaces, 88(2), 568-573 (2011)
Expression, purification and immunological characterization of the transforming protein E7, from cervical cancer-associated human papillomavirus type 16.
Fernando G J P, et al.
Clinical and Experimental Immunology, 115(3), 397-397 (1999)
Identification of a new 80 k isoform of phosphatidylinositol 4-phosphate 5-kinase from bovine brain.
Schmidt M, et al.
Febs Letters, 325(3), 201-204 (1993)
M Schmidt et al.
FEBS letters, 325(3), 201-204 (1993-07-05)
Phosphatidylinositol 4-phosphate 5-kinase is associated with bovine brain microsomes to an extent of approximately 65% of the total cellular enzyme activity. This membrane-associated kinase activity can be solubilized with Triton X-114. Following polyacrylamide gel electrophoresis in the presence of SDS
Extraction and identification of human platelet integral membrane proteins using Triton X-114.
P J Newman et al.
Thrombosis research, 27(2), 221-224 (1982-07-15)

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