MilliporeSigma
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93443

Sigma-Aldrich

Triton X-100 solution

BioUltra, for molecular biology, ~10% in H2O

Synonym(s):
Triton X-100, 4-(1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol solution, t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether
Linear Formula:
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
CAS Number:
Beilstein:
2315025
MDL number:
PubChem Substance ID:

Quality Level

grade

for molecular biology

description

non-ionic

product line

BioUltra

form

liquid

mol wt

micellar avg mol wt 80,000
average mol wt 625

concentration

~10% in H2O

aggregation number

100-155

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

refractive index

n20/D 1.349±0.002

pH

6.5-8.5 (25 °C)

CMC

0.2-0.9 mM (20-25°C)
0.23 mM

transition temp

cloud point 65 °C
pour point ~7 °C

density

1.01 g/mL at 20 °C

cation traces

Al: ≤1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤100 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Na: ≤100 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

absorption

cut-off at 310 nm

HLB

13.5

SMILES string

CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

InChI

1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3

InChI key

HNLXNOZHXNSSPN-UHFFFAOYSA-N

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General description

Triton X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

Application

Triton-X has been used:
  • for permeabilization of cells before techniques like indirect immunofluorescence and flow cytometry
  • As a component of lysis buffer used for homogenization of cells
  • As a component of blocking buffer in immunofluorescence staining
  • For the preparation of cells in double fluorescence staining

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Pictograms

CorrosionEnvironment

Signal Word

Danger

Hazard Statements

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

483.8 °F - closed cup

Flash Point(C)

251 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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