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Key Documents

S3014

Sigma-Aldrich

Sodium chloride

for molecular biology, DNase, RNase, and protease, none detected, ≥99% (titration)

Synonym(s):

Halite, NaCl

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About This Item

Linear Formula:
NaCl
CAS Number:
Molecular Weight:
58.44
Beilstein/REAXYS Number:
3534976
EC Number:
MDL number:
UNSPSC Code:
12352302
PubChem Substance ID:
NACRES:
NA.25

grade

for molecular biology

Quality Level

assay

≥99% (titration)

form

crystalline

impurities

DNAse, none detected
Endonuclease, none detected
NICKase, none detected
RNAse, none detected
protease, none detected

turbidity

≤3.5 NTU

pH

7

mp

801 °C (lit.)

solubility

H2O: 1 g/10 mL, clear, colorless

density

2.16 g/cm3 at 25 °C (77 °F)

anion traces

phosphate (PO43-): ≤5 ppm

cation traces

K: ≤50 ppm
≤5 ppm (Heavy Metals by ICP-OES)

suitability

suitable for Western blot
suitable for mass spectrometry
suitable for molecular biology

application(s)

cell analysis

foreign activity

DNase, RNase, and protease, none detected

SMILES string

[Na+].[Cl-]

InChI

1S/ClH.Na/h1H;/q;+1/p-1

InChI key

FAPWRFPIFSIZLT-UHFFFAOYSA-M

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Application

This product is designated as Molecular Biology grade and is suitable for molecular biology applications. It has been analyzed for the absence of nucleases and proteases.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

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Jules D P Valentin et al.
Journal of colloid and interface science, 552, 247-257 (2019-05-28)
Many materials used in the medical settings such as catheters and contact lenses as well as most biological tissues are not purely elastic, but rather viscoelastic. While substrate elasticity has been investigated for its influence on bacterial adhesion, the impact
Zlatko Kopecki et al.
Oncotarget, 6(34), 36426-36440 (2015-10-27)
Flightless I (Flii) is an actin remodeling protein that affects cellular processes including adhesion, proliferation and migration. In order to determine the role of Flii during carcinogenesis, squamous cell carcinomas (SCCs) were induced in Flii heterozygous (Flii+/-), wild-type and Flii
Ram S Puranam et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 35(23), 8866-8881 (2015-06-13)
We identified a family in which a translocation between chromosomes X and 14 was associated with cognitive impairment and a complex genetic disorder termed "Genetic Epilepsy and Febrile Seizures Plus" (GEFS(+)). We demonstrate that the breakpoint on the X chromosome
Avanti Gokhale et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 35(19), 7643-7653 (2015-05-15)
Dysbindin is a schizophrenia susceptibility factor and subunit of the biogenesis of lysosome-related organelles complex 1 (BLOC-1) required for lysosome-related organelle biogenesis, and in neurons, synaptic vesicle assembly, neurotransmission, and plasticity. Protein networks, or interactomes, downstream of dysbindin/BLOC-1 remain partially
Meriam Ayadi et al.
Oncotarget, 6(21), 18518-18533 (2015-06-05)
Most solid tumors contain a subfraction of cells with stem/progenitor cell features. Stem cells are naturally chemoresistant suggesting that chronic chemotherapeutic stress may select for cells with increased "stemness". We carried out a comprehensive molecular and functional analysis of six

Protocols

Selection of Poly(A)+ RNA by Oligo(dT)-Cellulose Chromatography

cAMP measurements are obtained using an ELISA assay (Harlow and Lane 1988). Commercial radio-immunoassays, or ELISA kits, to assay cAMP can be purchased from various manufacturers.

In Situ Hybridization of Whole-Mount Mouse Embryos with RNA Probes: Hybridization, Washes, and Histochemistry. This is a protocol describing how to perform in situ hybridization on whole mouse embryos. Here we describe the hybridization procedure, and the localization of the DIG-labeled RNA using a conjugate of anti-DIG Fab antibody and calf intestinal alkaline phosphatase. Enzyme activity of the reporter is detected by a color reaction, resulting in the formation of a water-insoluble purple/blue precipitate. Manipulating the Mouse Embryo - Third Edition

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