Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.
40% (w/w) solution in water is clear and colorless.
Trizma® base has been used:
- as a component of H buffer (cell dissociation buffer)
- for washing and saturation of wells in double sandwich ELISA immunoenzymatic technique
- as an assay buffer for reconstitution of extracted and dried protein samples
- to prepare Tris-HCl buffer that is used to stabilize proteins
- as a buffer to extract carotenoid from tubers
- as a component of sample buffer during protein extraction prior to western blotting
- as a component of sample buffer for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)
- to prepare simulated body fluid (SBF) for calcium phosphate (CaP) resorption assay
- as a buffer for polydopamine (PDA) deposition on stainless steel (SS) substrate
5, 10, 25, 50 kg in poly drum
25, 100, 250, 500 g in poly bottle
1 kg in poly bottle
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Easily compare specifications for Trizma products with the Trizma specification table.
Trizma is a registered trademark of Sigma-Aldrich Co. LLC