S0389

Sucrose

for molecular biology, ≥99.5% (GC)

• Synonim(y): α-D-Glc-(1→2)-β-D-Fru, α-D-Glucopyranosyl β-D-fructofuranoside, β-D-Fructofuranosyl-α-D-glucopyranoside, D(+)-Saccharose, Sugar
• Wzór empiryczny (zapis Hilla): C₁₂H₂₂O₁₁
• Numer CAS: 57-50-1
• Masa cząsteczkowa: 342.30
• Beilstein: 90825
• Numer WE: 200-334-9
• Numer MDL: MFCD00006626
• Kod UNSPSC: 12352201
• Identyfikator substancji w PubChem: 24899423
• NACRES: NA.31

Właściwości

• pochodzenie biologiczne: sugar cane
• Poziom jakości: 300
• klasa czystości: for molecular biology
• Próba: ≥99.5% (GC)
• Postać: crystals
• metody: HPLC: suitable; cryopreservation: suitable; electron microscopy: suitable; enzyme immunoassay: suitable; gas chromatography (GC): suitable; immunohistochemistry: suitable
• zanieczyszczenia: ≤0.1% free glucose
• kolor: white
• przydatny zakres pH: 5.5-7 (25 °C, 342 g/L)
• mp: 185-187 °C (lit.)
• rozpuszczalność: H₂O: soluble 50 g + 50 mL
• ślady kationów: heavy metals (as Pb): ≤5 ppm
• absorpcja: ≤0.15 at 280 at 50%; ≤0.20 at 260 at 50%
• przydatność: suitable for molecular biology
• Zastosowanie: agriculture; agriculture; life science and biopharma; metabolomics; sample preservation
• obecność zanieczyszczeń: DNase, RNase, protease, none detected
• temp. przechowywania: room temp
• ciąg SMILES: OC[C@H]1O[C@H](O[C@]2(CO)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@H](O)[C@@H](O)[C@@H]1O
• InChI: 1S/C12H22O11/c13-1-4-6(16)8(18)9(19)11(21-4)23-12(3-15)10(20)7(17)5(2-14)22-12/h4-11,13-20H,1-3H2/t4-,5-,6-,7-,8+,9-,10+,11-,12+/m1/s1
• Klucz InChI: CZMRCDWAGMRECN-UGDNZRGBSA-N

Opis

Opis ogólny

Sucrose, a disaccharide composed of glucose and fructose, serves as an energy source in various biological processes. In cell biology, sucrose acts as a cryoprotectant, preserving cell viability during freezing and thawing. It is also added as a supplement in culture media, aiding in maintaining osmotic balance and enhancing cell survival.In molecular biology, sucrose is crucial in gradient solutions for DNA and RNA purification and in density centrifugation for organelle separation.

Zastosowanie

Sucrose has been used:

• to perform cryoprotection for kidney tissues
• as a component in the solid germination medium to germinate pollen grains
• in the triphenyl-tetrazolium chloride (TTC) test to check the viability of pollen grains
• in the cryoprotection of fixed brain tissues for immunohistochemistry

Działania biochem./fizjol.

Sucrose is used as a transport and storage molecule in most plants due to its limited chemical reactivity. It can be used to prepare density gradients for the separation of cell/organelle. Sucrose can be used in the plant, insect, and bacterial culture media as a supplement. In addition, sucrose can also be used in several enzymatic assays.

Cechy i korzyści

• Ideal for Molecular Biology, Cell Biology and Biochemical research
• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
• Tested for DNase, RNase, and proteases
• Molecular biology grade sucrose, sourced from sugar cane

Inne uwagi

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)