Pular para o conteúdo
Merck
  • Experimental design-based isotope-dilution SPME-GC/MS method development for the analysis of smoke flavouring products.

Experimental design-based isotope-dilution SPME-GC/MS method development for the analysis of smoke flavouring products.

Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment (2017-09-09)
Anupam Giri, Zuzana Zelinkova, Thomas Wenzl
RESUMO

For the implementation of Regulation (EC) No 2065/2003 related to smoke flavourings used or intended for use in or on foods a method based on solid-phase micro extraction (SPME) GC/MS was developed for the characterisation of liquid smoke products. A statistically based experimental design (DoE) was used for method optimisation. The best general conditions to quantitatively analyse the liquid smoke compounds were obtained with a polydimethylsiloxane/divinylbenzene (PDMS/DVB) fibre, 60°C extraction temperature, 30 min extraction time, 250°C desorption temperature, 180 s desorption time, 15 s agitation time, and 250 rpm agitation speed. Under the optimised conditions, 119 wood pyrolysis products including furan/pyran derivatives, phenols, guaiacol, syringol, benzenediol, and their derivatives, cyclic ketones, and several other heterocyclic compounds were identified. The proposed method was repeatable (RSD% <5) and the calibration functions were linear for all compounds under study. Nine isotopically labelled internal standards were used for improving quantification of analytes by compensating matrix effects that might affect headspace equilibrium and extractability of compounds. The optimised isotope dilution SPME-GC/MS based analytical method proved to be fit for purpose, allowing the rapid identification and quantification of volatile compounds in liquid smoke flavourings.

MATERIAIS
Número do produto
Marca
Descrição do produto

Supelco
Conjunto de fibras para SPME de divinilbenzeno-carboxeno-polidimetilsiloxano (DVB/CAR/PDMS), needle size 24 ga, StableFlex fiber, for use with autosampler, fiber L 2 cm, pk of 3
Supelco
Conjunto de fibras para SPME de divinilbenzeno-carboxeno-polidimetilsiloxano (DVB/CAR/PDMS), needle size 24 ga, StableFlex fiber, for use with manual holder
Supelco
Conjunto de fibras para SPME de divinilbenzeno-carboxeno-polidimetilsiloxano (DVB/CAR/PDMS), StableFlex (1 cm), needle size 23 ga, Autosampler, pk of 3, plain gray hub
Supelco
Conjunto de fibras para SPME de divinilbenzeno-carboxeno-polidimetilsiloxano (DVB/CAR/PDMS), needle size 24 ga, StableFlex, for use with autosampler, fiber L 1 cm, pk of 3
Supelco
Conjunto de fibras para SPME de divinilbenzeno-carboxeno-polidimetilsiloxano (DVB/CAR/PDMS), StableFlex (2cm), needle size 23 ga, Autosampler, pk of 3, notched gray hub
Supelco
Conjunto de fibras para microextração em fase sólida (SPME) de polidimetilsiloxano (PDMS), df 100 μm(PDMS, for use with autosampler, needle size 23 ga
Supelco
Conjunto de fibras para microextração em fase sólida (SPME) de polidimetilsiloxano (PDMS), df 100 μm(PDMS, needle size 24 ga, for use with manual holder
Supelco
Conjunto de fibras para microextração em fase sólida (SPME) de polidimetilsiloxano (PDMS), df 100 μm(PDMS, for use with autosampler, needle size 24 ga
Supelco
Conjunto de fibras de SPME Carboxeno/polidimetilsiloxano (CAR/PDMS), df 75 μm(CAR/PDMS, for use with manual holder, needle size 24 ga
Sigma-Aldrich
Poly(dimethylsiloxane), viscosity 1.0 cSt (25 °C)
Supelco
SPME Fiber Assembly Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), df 65 μm(PDMS/DVB, needle size 24 ga, StableFlex, for use with autosampler
Supelco
Conjunto de fibras de SPME Carboxeno/polidimetilsiloxano (CAR/PDMS), df 85 μm(CAR/PDMS, needle size 23 ga, StableFlex, for use with autosampler
Supelco
Conjunto de fibras de SPME Carboxeno/polidimetilsiloxano (CAR/PDMS), df 85 μm(CAR/PDMS, needle size 24 ga, StableFlex, for use with autosampler
Supelco
Conjunto de fibras para microextração em fase sólida (SPME) de poliacrilato (PA), df 85 μm(PA, for use with autosampler, needle size 23 ga
Supelco
SPME Fiber Assembly Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), df 65 μm(PDMS/DVB, needle size 24 ga, for use with manual holder
Supelco
SPME Fiber Assembly Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), df 65 μm(PDMS/DVB, needle size 23 ga, PDMS/DVB StableFlex, for use with autosampler
Supelco
Conjunto de fibras de SPME Carboxeno/polidimetilsiloxano (CAR/PDMS), df 75 μm(CAR/PDMS, needle size 24 ga, for use with autosampler
Supelco
SPME Fiber Assembly Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), df 65 μm(PDMS/DVB, needle size 24 ga, for use with autosampler
Supelco
Conjunto de fibras de SPME Carboxeno/polidimetilsiloxano (CAR/PDMS), df 85 μm(CAR/PDMS, needle size 24 ga, for use with manual holder, StableFlex fiber
Supelco
SPME Fiber Assembly Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), df 65 μm(PDMS/DVB, needle size 24 ga, StableFlex, for use with manual holder
Supelco
Conjunto de fibras para microextração em fase sólida (SPME) de poliacrilato (PA), df 85 μm(PA, for use with autosampler, needle size 24 ga
Supelco
Conjunto de fibras para microextração em fase sólida (SPME) de polidimetilsiloxano (PDMS), df 100 μm(PDMS, for use with manual holder, needle size 23 ga
Supelco
Conjunto de fibras de SPME Carboxeno/polidimetilsiloxano (CAR/PDMS), df 75 μm(CAR/PDMS, needle size 23 ga, for use with autosampler
Supelco
SPME Fiber Assembly Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), df 65 μm(PDMS/DVB, for use with autosampler, needle size 23 ga
Supelco
SPME StableFlex fiber assortment kit, for use with manual holder
Supelco
SPME fiber assembly, Carbowax-Polyethylene Glycol (PEG) Coating, needle size 23 ga, df 60 μm(PEG, for use with autosampler
Supelco
SPME StableFlex fiber assortment kit, needle size 23 ga, StableFlex fiber, for use with autosampler
Supelco
Conjunto de fibras de SPME Carboxeno/polidimetilsiloxano (CAR/PDMS), df 75 μm(CAR/PDMS, needle size 23 ga, for use with manual holder
Supelco
Conjunto de fibras para SPME de divinilbenzeno-carboxeno-polidimetilsiloxano (DVB/CAR/PDMS), for use with autosampler, needle size 23 ga, metal alloy fiber, fiber L 2 cm
Supelco
Conjunto de fibras para microextração em fase sólida (SPME) de poliacrilato (PA), df 85 μm(PA, for use with manual holder, needle size 24 ga