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Key Documents

SRP6556

Sigma-Aldrich

Thrombin Active (High Activity) from bovine plasma

≥98% (SDS-PAGE), recombinant, lyophilized

Sinônimo(s):

Activated Factor IIa

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About This Item

Código UNSPSC:
12352202
NACRES:
NA.32

product name

Thrombin Active (High Activity) from bovine plasma, ≥98% (SDS-PAGE)

fonte biológica

bovine plasma

Ensaio

≥98% (SDS-PAGE)

forma

lyophilized

potência

>1500 units/mg

peso molecular

37 kDa

embalagem

pkg of 10,000 units
pkg of 100,000 units
pkg of 1000 units

nº de adesão UniProt

Condições de expedição

wet ice

temperatura de armazenamento

−20°C

Informações sobre genes

bovine ... F2(280685)

Descrição geral

Thrombin is a coagulation protein and a serine protease (EC 3.4.21.5) that catalyzes many coagulation-related reactions.

Ações bioquímicas/fisiológicas

Thrombin enzyme (Activated Factor IIa) is an important clotting promoter that controls the transformation of soluble fibrinogen to insoluble active fibrin strands. Thrombin is a coagulation protein and a serine protease (EC 3.4.21.5) that catalyzes many coagulation-related reactions. Thrombin triggers factor-XI, factor-V, Factor-XIII and factor-VIII. Thrombin endorses platelet activation, using activation of protease-activated receptors on the platelet. As a result of its high proteolytic specificity, thrombin has become an important biochemical protein. The thrombin cleavage site (Leu-Val-Pro-Arg-Gly-Ser) is widely used in linker regions of recombinant fusion protein constructs. After the purification of the fusion protein, thrombin is used to cleave between the Arginine and Glycine residues of the cleavage site, efficiently removing the purification tag from the protein of interest with a high degree of specificity. Thrombin enzyme (Activated Factor IIa) also participates in inflammation and has mitogenic role on vascular cells. It is also involved in the activation of protein C which is needed for the inactivation of many procoagulant enzymes.

forma física

Sterile filtered and lyophilized with Mannitol and Sodium Chloride.

Reconstituição

Reconstitute in sterile water (100 U/mL) with 0.9% NaCl. It forms a clear solution.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

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The CUG codon is decoded in vivo as serine and not leucine in Candida albicans.
Santos MA and Tuite MF
Nucleic Acids Research, 23, 1481-1486 (1995)
Exogenous thrombin delivery promotes collateral capillary arterialization and tissue reperfusion in the murine spinotrapezius muscle ischemia model.
Bruce AC and Peirce SM
Microcirculation (New York, N.Y. : 1994), 19, 143-154 (2012)
Effects of coagulation factor deficiency on plasma coagulation kinetics determined via thrombelastography: critical roles of fibrinogen and factors II, VII, X and XII.
Nielsen VG, et al.
Acta Anaesthesiologica Scandinavica, 49, 222-231 (2005)
A dual thrombin receptor system for platelet activation.
Kahn ML, et al.
Nature, 394, 690-694 (1998)
Biophysical investigation of GpIbalpha binding to thrombin anion binding exosite II.
Sabo TM and Maurer MC
Biochemistry, 48, 7110-7122 (2009)

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