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P4978

Sigma-Aldrich

Phosphatase, Alkaline from calf intestine

buffered aqueous glycerol solution

Sinônimo(s):

CIAP, CIP, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

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About This Item

Número CAS:
9001-78-9
Número da licença da enzima:
3.1.3.1 (BRENDA, IUBMB)
Número MDL:
MFCD00131849
Código UNSPSC:
12352204
eCl@ss:
42010105
NACRES:
NA.53

grau

for molecular biology

forma

buffered aqueous glycerol solution

peso molecular

~80 kDa

concentração

≥10,000 units/mL

nº de adesão UniProt

P19111

atividade externa

DNase, RNase, none detected

Condições de expedição

wet ice

temperatura de armazenamento

−20°C

Informações sobre genes

cow ... ALPI(280993)

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Descrição geral

Alkaline phosphatase is a phosphomonoesterase that catalyzes the cleavage of terminal phosphates from nucleic acids. The free 5′-OH can be phosphorylated with polynucleotide kinase and γ32P-ATP to produce γ32P-end-labeled nucleic acids. Linearized cloning vectors can be prevented from recircularizing by dephosphorylation with alkaline phosphatase.

Aplicação

Commonly used to remove the 5′-terminal phosphate from nucleic acids during molecular cloning reactions.

Componentes

Alkaline phosphatase is provided in a solution of 10 mM Tris-HCl (pH 8.2), 50 mM KCl, 1 mM MgCl2, and 0.1 mM ZnCl2, in 50% (w/v) glycerol. 10X CIP Buffer, Product Number C3225, is included.

Definição da unidade

One unit will hydrolyze 1 μmole of p-nitrophenyl phosphate per min at 37 °C.

Outras notas

Alkaline phosphatase in 5 mM EDTA (pH 8) will be irreversibly heat inactivated at 75 °C for 10 minutes.

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LIG1

DNA Ligation Kit

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

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Protocolos

Diethanolamine Assay for the Enzymatic Assay of Alkaline Phosphatase (EC 3.1.3.1)

Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)

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