P4252
Phosphatase, Alkaline from Escherichia coli
ammonium sulfate suspension, 30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)
Sinônimo(s):
Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
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About This Item
Produtos recomendados
fonte biológica
Escherichia coli
Nível de qualidade
Formulário
ammonium sulfate suspension
atividade específica
30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)
temperatura de armazenamento
2-8°C
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Aplicação
Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P4252 has been used for bulk digestion of tRNA . Product P4252 is provided in the ratio of 30-90 units/mg of protein.
Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P4252 is an ammonium sulfate suspension in glycine buffer.
Ações bioquímicas/fisiológicas
Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters.
Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters.
Atenção
Presence of 0.1% SDS in the alkaline phosphatase assays will inhibit any nuclease contamination in the sample.
Definição da unidade
One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 10.4 at 37 °C.
forma física
Suspension in 2.5 M (NH4)2SO4
Nota de análise
The activity at pH 8.0 at 25 °C is approximately the same as at pH 10.4 at 37 °C.
Inibidor
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Resp. Sens. 1
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
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Artigos
Instructions for working with enzymes supplied as ammonium sulfate suspensions
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