D7291

Deoxyribonuclease I RNase-free solution from bovine pancreas

• Sinônimo(s): DNase I, Deoxyribonuclease I, pancreatic DNase I, pancreatic deoxyribonuclease I
• Número CAS: 9003-98-9
• Número da licença da enzima: 3.1.21.1 (BRENDA, IUBMB)
• Número CE: 232-667-0
• Número MDL: MFCD00130918
• Código UNSPSC: 12352204
• NACRES: NA.53

Propriedades

• fonte biológica: bovine pancreas
• Nível de qualidade: 200
• grau: for molecular biology
• Formulário: buffered aqueous glycerol solution
• atividade específica: 10000 units/mg protein
• peso molecular: 29.1 kDa
• concentração: 5—15 mg protein/mL
• técnica(s): DNA extraction: suitable
• adequação: suitable for molecular biology
• nº de adesão UniProt: P00639
• aplicação(ões): cell analysis
• atividade externa: RNase, none detected
• temperatura de armazenamento: −20°C
• Informações sobre genes: cow ... DNASE1(282217)

Descrição

Descrição geral

Deoxyribonuclease I digests single- and double-stranded DNA to a mixture of mono and oligonucleotides carrying 5′phosphates and 3′OH termini. This catalytic activity is divalent ion-dependent. In the presence of Mg²⁺, DNase I hydrolyzes each strand of double-stranded DNA randomly and independently. In the presence of Mn²⁺, both strands can be cleaved.

Aplicação

Deoxyribonuclease I RNase-free solution from bovine pancreas has been used to digest DNA from various samples.

Used in molecular biology applications for removing DNA during RNA purification, for preparing DNA for nick translation, and for DNA-protein interaction analysis by footprinting methods.

Componentes

DNase I is provided in a solution of 50% (v/v) glycerol, 20 mM sodium acetate (pH 6.5), 5 mM CaCl2, and 0.1 mM PMSF.

Definição da unidade

One unit will cause a ΔA₂₆₀ of 0.001 per min per mL reaction mixture using calf thymus DNA as substrate.

Outras notas

RNA treated with this DNase I should not be used to generate a cDNA library or used for RT-PCR. For these sensitive applications, it is recommended to use DNase I, Amplification Grade , Catalog Number AMP-D1.

Informações de segurança

• Pictogramas: GHS08
• Palavra indicadora: Danger
• Frases de perigo: H334
• Declarações de precaução: P261 - P284 - P501
• Classificações de perigo: Resp. Sens. 1
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)