R0884

T7 RNA Polymerase

recombinant, expressed in E. coli, buffered aqueous solution

• Sinônimo(s): RNA Polymerase T7, RNA Polymerase, T7 from E. coli HMS 174/pAR1219
• Número CAS: 9014-24-8
• Número da licença da enzima: 2.7.7.6 (BRENDA, IUBMB)
• Número MDL: MFCD00132197
• Código UNSPSC: 12352204

Propriedades

• recombinante: expressed in E. coli
• grau: for molecular biology
• forma: buffered aqueous solution
• peso molecular: 98.8 kDa
• concentração: 10,000-50,000 U/mL
• nº de adesão UniProt: P00573
• atividade externa: DNase and RNase, none detected
• temperatura de armazenamento: −20°C
• Informações sobre genes: bacteriophage T7 ... T7p07(1261050)

Descrição

Descrição geral

T7 RNA polymerase is highly specific for the bacteriophage T7 promoter and terminator sequences. It is extensively used to prepare RNA transcripts for stuctural and metabolic studies. The RNA transcripts can be converted to probes for sensitive hybridization detection studies. T7 polymerase and dideoxynucleotides can be used to directly sequence DNA.

Componentes

T7 RNA Polymerase is supplied as a solution of 100 mM NaCl, 50 mM Tris-HCl (pH 7.9), 0.1 mM EDTA, 0.1% Triton X-100, 1 mM DTT, and 50% (v/v) glycerol.

Definição da unidade

One unit will catalyze the incorporation of 1 nmol of rNTP into acid-precipitable material in 60 min at 37°C.

Nota de análise

Activity assay: 40 mM Tris-HCl, pH 7.9, 6 mM MgCl₂, 4 mM spermidine, 10 mM DTT, 0.5 μM each rNTP + 10 μCi α-³²P-UTP, 3-10 units of enzyme, and 1 μg of a 350 bp template are incubated for 10 min at 37°C in a total volume of 100 μl. Typical results are ≥50% incorporation of labeled nucleotide into ≥90% full-length transcript.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable