R0884
T7 RNA Polymerase
recombinant, expressed in E. coli, buffered aqueous solution
Sinônimo(s):
RNA Polymerase T7, RNA Polymerase, T7 from E. coli HMS 174/pAR1219
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About This Item
Número CAS:
Número MDL:
Código UNSPSC:
12352204
Produtos recomendados
recombinante
expressed in E. coli
grau
for molecular biology
Formulário
buffered aqueous solution
peso molecular
98.8 kDa
concentração
10,000-50,000 U/mL
nº de adesão UniProt
atividade externa
DNase and RNase, none detected
temperatura de armazenamento
−20°C
Informações sobre genes
bacteriophage T7 ... T7p07(1261050)
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Descrição geral
T7 RNA polymerase is highly specific for the bacteriophage T7 promoter and terminator sequences. It is extensively used to prepare RNA transcripts for stuctural and metabolic studies. The RNA transcripts can be converted to probes for sensitive hybridization detection studies. T7 polymerase and dideoxynucleotides can be used to directly sequence DNA.
Componentes
T7 RNA Polymerase is supplied as a solution of 100 mM NaCl, 50 mM Tris-HCl (pH 7.9), 0.1 mM EDTA, 0.1% Triton X-100, 1 mM DTT, and 50% (v/v) glycerol.
Definição da unidade
One unit will catalyze the incorporation of 1 nmol of rNTP into acid-precipitable material in 60 min at 37°C.
Nota de análise
Activity assay: 40 mM Tris-HCl, pH 7.9, 6 mM MgCl2, 4 mM spermidine, 10 mM DTT, 0.5 μM each rNTP + 10 μCi α-32P-UTP, 3-10 units of enzyme, and 1 μg of a 350 bp template are incubated for 10 min at 37°C in a total volume of 100 μl. Typical results are ≥50% incorporation of labeled nucleotide into ≥90% full-length transcript.
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Descrição
Preços
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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Characterization of T7-specific ribonucleic acid polymerase. 1. General properties of the enzymatic reaction and the template specificity of the enzyme.
M Chamberlin et al.
The Journal of biological chemistry, 248(6), 2235-2244 (1973-03-25)
Byung-Chun Kim et al.
Journal of microbiology and biotechnology, 23(2), 189-194 (2013-02-16)
In a study of hydrogen-producing bacteria, strain T4384 was isolated from rice field samples in the Republic of Korea. The isolate was identified as Enterobacter sp. T4384 by phylogenetic analysis of 16S rRNA and rpoB gene sequences. Enterobacter sp. T4384
Ran Furman et al.
FEBS letters, 587(6), 614-619 (2013-02-19)
Transcription factor DksA contains a four-Cys Zn(2 +)-finger motif thought to be responsible for structural integrity and the relative disposition of its domains. Pseudomonas aeruginosa encodes an additional DksA paralog (DksA2) that is expressed selectively under Zn(2+) limitation. Although DksA2
Looking for a promoter in 3D.
Vladimir Svetlov et al.
Nature structural & molecular biology, 20(2), 141-142 (2013-02-06)
Yuko Murayama et al.
Acta crystallographica. Section F, Structural biology and crystallization communications, 69(Pt 2), 174-177 (2013-02-07)
DNA-dependent RNA polymerase (RNAP) synthesizes RNA complementary to the template DNA. During transcript elongation, RNAP often undergoes backward translocation ('backtracking') by dissociating the 3' end of the nascent RNA transcript from the template DNA. While the backtracked state of RNAP
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