10745782001
Roche
DNA, lambda
from bacteriophage lambda cl857 Sam 7
Sinônimo(s):
lambda dna
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About This Item
Código UNSPSC:
41106310
Produtos recomendados
Nível de qualidade
forma
solution
peso molecular
32,300 kDa
embalagem
pkg of 1 mL
fabricante/nome comercial
Roche
condição de armazenamento
avoid repeated freeze/thaw cycles
concentração
250 μg/mL
Impurezas
RNA, none detected
nuclease, none detected
cor
colorless
solubilidade
water: miscible
Condições de expedição
dry ice
temperatura de armazenamento
−20°C (−15°C to −25°C)
Descrição geral
The DNA of bacteriophage lambda contains 48,502 nucleotide pairs forming a linear duplex. The 5′ ends of this DNA molecule have self-complementary single-stranded protrusions of 12 nucleotides that can anneal to form circles. Upon entry into the host cell, the DNA circularizes in the presence of DNA ligase. Many such circles are generated during the early lytic infection. At a later stage in replication, these individual circles/rings are converted to linear concatemers by a process called rolling-circle replication. The linear concatemers are cleaved during packaging to form monomeric chromosomal units by the enzyme DNA terminase.
Aplicação
DNA, lambda has been used to spike peripheral blood leukocytes (PBL) before genomic DNA isolation. It has also been used in the preparation of biotinylated double-stranded DNA (dsDNA) substrate.
Qualidade
The DNA shows the typical cleavage pattern for wild type λDNA in gel electrophoresis after cleavage with restriction endonucleases EcoR I and Hind III.
Sequência
Chain Length 48,502 bp
forma física
Solution, 250 μg/ml, in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0, ready-to-use
Armazenamento e estabilidade
Store at -15 to -25°C until use; once thawed, store at 2-8 °C.
Outras notas
For life science research only. Not for use in diagnostic procedures.
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
nwg
Ponto de fulgor (°F)
No data available
Ponto de fulgor (°C)
No data available
Certificados de análise (COA)
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