D2916

Lambda DNA Mixed Digest

for DNA electrophoresis

• Código UNSPSC: 12352200
• NACRES: NA.25

Propriedades

• Nível de qualidade: 100; 200
• Formulário: buffered aqueous solution
• uso: 100 uses (0.1 mg size); 20 uses (20 μg size)
• adequação: suitable for electrophoresis (DNA)
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

Sigma′s Lambda DNA Mixed Digest contains 10 fragments from 1,503 − 48,502 bp. Six ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Aplicação

Lambda DNA Mixed Digest has been used for DNA electrophoresis.

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

Componentes

Sigma′s Lambda DNA Mixed Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Nota de preparo

Bacteriophage lambda DNA was completely digested with by Apa I, Kpn I, Xba I and Xho I in separate reactions, sticky ends filled with DNA polymerase I Klenow (to prevent reannealing), mixed with uncut bacteriophage lambda DNA, and then purified by phenol extraction.

Outras notas

For optimal resolution, the recommended agarose gel concentration is 0.4%.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable