D9281

Lambda DNA EcoR I Hind III Digest

for DNA electrophoresis

• Código UNSPSC: 41141827
• NACRES: NA.25

Propriedades

• Nível de qualidade: 100; 200
• forma: liquid
• uso: 100 uses (0.1 mg size); 500 uses (0.5 mg size)
• adequação: suitable for electrophoresis (DNA)
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

Sigma′s Lambda DNA EcoR I Hind III Digest contains 13 fragments from 125 – 21,226 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Aplicação

Lambda DNA EcoR I Hind III Digest has been used as DNA markers for comparing the molecular weights of polymerase chain reaction (PCR) amplified products.

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

Componentes

Sigma′s Lambda DNA EcoR I Hind III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Outras notas

Contains 13 fragments, 125–21,226 bp

For optimal resolution, the recommended agarose gel concentration is 0.7%.

Nota de preparo

Bacteriophage lambda DNA was digested with EcoR I and Hind III to completion, then purified by phenol extraction.

Informações de segurança

• Pictogramas: GHS07
• Palavra indicadora: Warning
• Frases de perigo: H317
• Declarações de precaução: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Classificações de perigo: Skin Sens. 1
• Código de classe de armazenamento: 13 - Non Combustible Solids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable