D9655

pBR322 Hae III Digest

for DNA electrophoresis

• Código UNSPSC: 12352202
• NACRES: NA.25

Propriedades

• Nível de qualidade: 100
• Formulário: liquid
• uso: 1000 uses (0.5 mg size); 200 uses (0.1 mg size)
• adequação: suitable for electrophoresis (DNA)
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

Sigma′s pBR322 Hae III Digest contains 22 fragments from 8 - 587 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Aplicação

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

Componentes

Sigma′s pBR322 Hae III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Outras notas

Contains 22 fragments, 8–587 bp

For optimal resolution, the recommended agarose gel concentration is 2.5.

Nota de preparo

Plasmid pBR322 DNA was digested with Hae III to completion, then purified by phenol extraction.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable