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D3812

Sigma-Aldrich

DirectLoad 50 bp DNA Step Ladder

ready-to-use marker for DNA electrophoresis

Sinônimo(s):

DirectLoad 50 bp DNA Marker, Ready-to-use 50 bp DNA Marker, Ready-to-use DirectLoad 50 bp DNA Marker, Ready-to-use DirectLoad 50 bp DNA Step Ladder

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About This Item

Código UNSPSC:
41105335
NACRES:
NA.25

Nível de qualidade

forma

(liquid)

uso

sufficient for 100 loads

adequação

suitable for electrophoresis (DNA)

temperatura de armazenamento

−20°C

Descrição geral

Sigma′s DirectLoad 50 bp DNA Step Ladder contains 17 blunt-ended fragments consisting of 50 bp repeats from 50 to 500 bp, 100 bp repeats from 600 to 900 bp, and 1 kb repeats from 1 to 3 kb. Ten ul of the ladder can be loaded directly in a single lane on an agarose or polyacrylamide gel.

Aplicação

DirectLoad 50 bp DNA Step Ladder has been used for cell lysis and genomic cleavage detection assay.
Suitable for size determination of PCR-generated DNA fragments with either agarose or polyacrylamide gels.

Características e benefícios

  • Ready-to-load
  • Easy-to-use
  • Popular band sizes

Componentes

Sigma′s DirectLoad 50 bp DNA Step Ladder is provided in a gel-loading solution of 5% glycerol, 4.2 mM EDTA, 0.083% orange G and 0.0125% xylene cyanol.

Outras notas

For optimal resolution, the recommended agarose gel concentration is 2.0%.

Informações legais

DirectLoad is a trademark of Sigma-Aldrich Co. LLC

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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Artigos

We offers a variety of markers that aid size determination of samples separated by agarose and/or polyacrylamide gel electrophoresis. These products include markers for DNA, PCR fragments and RNA and can be concurrently run with the samples. All the markers stain well with common nucleic acid stains.

Protocolos

When using hot start Taq DNA polymerase, the enzyme remains inactive until heated. Hot Start DNA polymerase control is achieved by chemical or antibody modification of the enzyme.

Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

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