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Key Documents

GE17-5282-02

GSTrap High Performance

Cytiva 17-5282-02, pack of 5 × 5 mL

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About This Item

Código UNSPSC:
23151817
NACRES:
NA.56

ligand

glutathione

embalagem

pack of 5 × 5 mL

fabricante/nome comercial

Cytiva 17-5282-02

Parâmetros

<20 mL/min flow rate
42 psi (H2O at room temperature.)

tamanho do leito

16 mm × 25 mm

volume do leito

5 mL

D.I. da coluna

16 mm

matriz

highly cross-linked 6% agarose

Tamanho médio da partícula

34 μm

cleaning

3-12

working range

3-12

capacidade

>7 mg/mL binding capacity (medium)

Descrição geral

GSTrap HP columns are prepacked with Glutathione Sepharose High Performance medium and provide high-resolution, one-step purifications of glutathione S-Transferase (GST) tagged proteins.

GSTrap HP 1-ml and 5-ml columns are prepacked with Glutathione Sepharose High Performance medium, which provides high-resolution purifications, thus a more concentrated sample. GST-tagged proteins are purified from pretreated bacterial lysates by affinity chromatography using immobilized glutathione, such as Glutathione Sepharose High Performance. GST-tagged proteins are bound to the affinity medium, and impurities are removed by washing with binding buffer.

GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Características e benefícios

  • High resolution purification of GST-tagged proteins.
  • Mild elution conditions preserving protein antigenicity and function.
  • Fast, one-step purification.
  • Easy to use with syringe, pump or a chromatography system.

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Outras notas

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Informações legais

GSTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

Pictogramas

Flame

Palavra indicadora

Warning

Frases de perigo

Código de classe de armazenamento

3 - Flammable liquids


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Artigos

This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from GE Healthcare.

This page shows troubleshooting methods for purification of GST-tagged proteins using GE Healthcare products.

This page describes characteristics of Glutathione Sepharose and HiTrap Benzamidine FF (high sub) media and columns from GE Healthcare.

This page describes troubleshooting strategies in purification of GST-tagged proteins using GE Healthcare products.

Protocolos

This page shows how to purify GST-tagged proteins using GSTrap HP, GSTrap FF and GSTrap 4B columns from GE Healthcare.

Characteristics of Glutathione Sepharose® Products

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica