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Key Documents

GE28-4055-00

GST MultiTrap 4B

Cytiva 28-4055-00

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About This Item

Código UNSPSC:
12161501
NACRES:
NA.32

ligand

glutathione

Materiais

polypropylene and polyethylene

fabricante/nome comercial

Cytiva 28-4055-00

embalagem

pkg of 4 x 96-well

tamanho

127.8 mm × 85.5 mm × 30.6 mm

matriz

4% agarose

tamanho de partícula

45-165 μm

diâmetro médio

90 μm

cleaning

4-13

working range

4-13

capacidade

>25 mg binding capacity (horse liver GST/ml medium)

Descrição geral

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
GST MultiTrap FF and GST MultiTrap 4B are prepacked, 96-well filter plates for reproducible, high-throughput screening and rapid parallel purification of glutathione S-transferase (GST) tagged proteins.

Aplicação

GST MultiTrap FF and GST MultiTrap 4B are prepacked, 96-well filter plates for reproducible, high-throughput screening and purification of glutathione S-transferase (GST) tagged proteins.

Purification of up to 0.5 mg of GST-tagged Proteins/well directly from unclarified cell lysate can be achieved using GST MultiTrap filter plates. Direct purification of GST-tagged Proteins from unclarified lysate shortens handling time and minimizes degradation of sensitive target Proteins. The 96-well plate format gives great flexibility, both when working with automated robotic systems and manually using centrifugation or vacuum. Consistent well-to-well and plate-to-plate performance ensures high reproducibility.

GST MultiTrap FF is prepacked with Glutathione Sepharose 4 Fast Flow, while GST MultiTrap 4B is prepacked with Glutathione Sepharose 4B.

Características e benefícios

  • 96-well filter plate format for high-throughput screening and parallel purification of GST-tagged* Proteins.
  • Highly reproducible well-to well and plate-to-plate results.
  • High purity in one step, high chemical stability. Load unclarified samples directly to the wells.
  • Wells are prepacked with Glutathione Sepharose media for convenience.
  • Easy and predictable scale-up using prepacked GSTrap FF, GSTPrep FF 16/10, and GSTrap 4B columns.

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informações legais

GSTPrep is a trademark of Cytiva
GSTrap is a trademark of Cytiva
MultiTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

Pictogramas

Flame

Palavra indicadora

Warning

Frases de perigo

Código de classe de armazenamento

3 - Flammable liquids


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Slide 1 of 4

1 of 4

Artigos

This page shows troubleshooting methods for purification of GST-tagged proteins using GE Healthcare products.

This page describes characteristics of Glutathione Sepharose and HiTrap Benzamidine FF (high sub) media and columns from GE Healthcare.

This page describes troubleshooting strategies in purification of GST-tagged proteins using GE Healthcare products.

Protocolos

This page how to remove GST tags by enzymatic cleavage using GE Healthcare products.

This page shows how to solubilize membrane proteins with products from GE Healthcare.

Characteristics of Glutathione Sepharose® Products

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

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