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GE17-0756-01

Glutathione Sepharose™ 4B

Cytiva 17-0756-01, pack of 10 mL

Sinônimo(s):

affinity chromatography resin, GST-tagged proteins purification,

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About This Item

Código UNSPSC:
41106500
NACRES:
NA.56

ligand

glutathione

embalagem

pack of 10 mL

fabricante/nome comercial

Cytiva 17-0756-01

matriz

4% agarose

tamanho de partícula

45-165 μm

diâmetro médio

90 μm

cleaning in place

4-13

working range

4-13

capacidade

>25 mg binding capacity (horse liver GST/ml medium)

Categorias relacionadas

Descrição geral

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
Glutathione Sepharose™ 4B is designed for high capacity single-step purification of Glutathione S-Transferase (GST) tagged fusion proteins.

GST-tagged proteins and other glutathione S-transferases or glutathone-dependent proteins can be purified directly from bacterial lysates using Glutathione Sepharose™ 4B. Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity.

Características e benefícios

  • High capacity single step purification of recombinant glutathione S-transferase (GST) tagged proteins
  • Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity and functionality.

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informações legais

Sepharose is a trademark of Cytiva

Pictogramas

Flame
GHS02

Palavra indicadora

Warning

Frases de perigo

H226

Código de classe de armazenamento

3 - Flammable liquids

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Artigos

Troubleshooting of Problems Associated with the Expression and Growth of GST-tagged Proteins

This page addresses common problems associated with the expression and growth of GST-tagged proteins using GE Healthcare products.

General Considerations for Purification of GST-tagged Proteins

This page describes general considerations for purification of GST-tagged proteins using GE Healthcare products.

General Considerations for Purification of GST-tagged Protein

This page describes general considerations for purification of GST-tagged proteins using GE Healthcare products.

Troubleshooting of Cleavage Methods

This page shows troubleshooting strategies for cleavage methods using GE Healthcare products.

Ver tudo

Protocolos

GST Detection Module with CDNB Enzymatic Assay

This page describes enzymatic detection of GST-tagged proteins with the GST Detection Module from GE Healthcare.

Purification using Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, and Glutathione Sepharose® 4B

This page shows how to purify GST-tagged proteins using Glutathione Sepharose from GE Healthcare.

Removal of GST Tag by Enzymatic Cleavage

This page how to remove GST tags by enzymatic cleavage using GE Healthcare products.

Pull-down assays

This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from GE Healthcare.

Ver tudo

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