GE28-9523-60

GST GraviTrap^™

Cytiva 28-9523-60

• Código UNSPSC: 41106501
• NACRES: NA.56

Propriedades

• ligand: glutathione
• embalagem: pkg of 10 ea
• fabricante/nome comercial: Cytiva 28-9523-60
• volume do leito: 2 mL
• D.I. da coluna: 15 mm
• Matriz: 4% agarose
• tamanho de partícula: 45-165 μm
• diâmetro médio: 90 μm
• cleaning: 4-13
• Faixa de trabalho: 4-13
• capacidade: ≥20 mg binding capacity(recombinant glutathione S-transferase (rGST)); >25 mg binding capacity (horse liver GST/ml medium)

Descrição

Descrição geral

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.

GST GraviTrap^™ contains 10 disposable columns prepacked with 2 mL of Glutathione Sepharose^™ 4B, sufficient for purification of up to 20 mg of fusion protein.

Aplicação

GST GraviTrap^™ contains 10 disposable columns prepacked with 2 mL of Glutathione Sepharose^™ 4B, sufficient for purification of up to 20 mg of fusion Protein. GST-tagged Proteins are recovered under mild elution conditions (10 mM glutathione), which preserves functionality of the Proteins. The binding capacity of prepacked GST GraviTrap^™ columns, which can be greater than 10 mg Protein/mL medium, varies with the nature of the tagged Protein and the exact binding conditions used. For increased convenience, GST Buffer Kit contains stock solutions of the buffers needed for purification of GST-tagged Proteins.

Características e benefícios

• Convenient, prepacked gravity-fLow columns.
• Allows direct purification of both clarified and unclarified cell lysates.
• Yields up to 20 mg of pure Proteins per column.
• Yields up to 20 mg of pure Proteins per column

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informações legais

GraviTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

Informações de segurança

• Pictogramas: GHS02
• Palavra indicadora: Warning
• Frases de perigo: H226
• Declarações de precaução: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Código de classe de armazenamento: 3 - Flammable liquids