GE28-9852-55
Protein G GraviTrap™
Cytiva 28-9852-55, pack of 10 × 1 mL
Sinônimo(s):
GraviTrap for Protein G, High Performance GraviTrap, Protein G GraviTrap
About This Item
Produtos recomendados
embalagem
pack of 10 × 1 mL
fabricante/nome comercial
Cytiva 28-9852-55
condição de armazenamento
(20% Ethanol)
volume do leito
1 mL
capacidade
~20 mg binding capacity (human IgG)(Protein binding capacity is protein-to-protein dependent.)
temperatura de armazenamento
2-8°C
Categorias relacionadas
Descrição geral
Protein G GraviTrap™ are prepacked gravity-flow columns designed for fast and efficient manual purification of monoclonal and polyclonal antibodies, antibody fragments from cell culture supernatant, and biological fluids. The antibodies are simply captured with high specificity on protein G ligands in gravity-flow columns. You do not need any other instrument with this protocol because the entire process relies on the flow of gravity. The yield varies from 20 to 50 mg of purified antibodies depending on the ligand you use. The columns are reusable up to five times.
Aplicação
Características e benefícios
- Prepacked gravity-flow columns provide convenience, save time, and increase the reliability of your results.
- Purification of antibodies in 30 min allows you to quickly obtain and store your target protein in order to avoid the inherent dangers of protein instability and degradation associated with slow and long purification runs.
- High purity and recovery with purity >95% in one step and recovery typically 70% to 80%.
- High binding capacity such as Protein G GraviTrap™ ~ 20 mg human IgG/column
Armazenamento e estabilidade
Nota de análise
Informações legais
Palavra indicadora
Warning
Frases de perigo
Declarações de precaução
Código de classe de armazenamento
3 - Flammable liquids
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Artigos
This page shows how to separate IgG antibodies by affinity chromatography using Protein G GraviTrap from GE Healthcare.
This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.
Protocolos
This page shows how to separate IgG antibodies by affinity chromatography using Protein G GraviTrap from GE Healthcare
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