Pular para o conteúdo
Merck
Todas as fotos(1)

Key Documents

View All Documentation

P3296

Millipore

Protein G Sepharose™, Fast Flow

recombinant, expressed in E. coli, aqueous ethanol suspension

Sinônimo(s):

Protein G-Agarose, Fast Flow from Streptococcus sp.

Faça loginpara ver os preços organizacionais e de contrato
Todas as fotos(1)

About This Item

Número MDL:
MFCD00165580
Código UNSPSC:
41106500
NACRES:
NA.56

recombinante

expressed in E. coli

Nível de qualidade

200

forma

aqueous ethanol suspension

classe(s) química(is) do analito

proteins (Immunoglobulins of various mammalian species)

Extensão da rotulagem

~2 mg per mL

técnica(s)

affinity chromatography: suitable

matriz

Sepharose 4B Fast Flow

ativação da matriz

cyanogen bromide

ligação da matriz

amino

espaçador de matriz

1 atom

temperatura de armazenamento

2-8°C

Procurando produtos similares? Visita Guia de comparação de produtos

Categorias relacionadas

Descrição geral

Protein G is an immunoglobulin (IgG)-binding bacterial cell wall protein isolated from group G streptococcal strain, G-148. This protein can be extracted from the cells by papain digestion and purified by the sequential use of ion-exchange chromatography on DEAE-Sephadex, affinity chromatography on Sepharose-coupled human IgG, and gel chromatography on Sephadex G-200. The binding between protein G and various polyclonal and monoclonal IgG is basically pH dependent between 2.8 and 10, with the strongest binding at pH 4 and 5, and weakest at pH 10. It acts as a powerful reagent for the detection of IgG.
Protein G is an immunoglobulin (IgG)-binding bacterial cell wall protein isolated from group G streptococcal strain, G-148. This protein can be extracted from the cells by papain digestion and purified by the sequential use of ion-exchange chromatography on DEAE-Sephadex, affinity chromatography on Sepharose-coupled human IgG, and gel chromatography on Sephadex G-200. The binding between protein G and various polyclonal and monoclonal IgG is basically pH dependent between 2.8 and 10, with the strongest binding at pH 4 and 5, and weakest at pH 10. It acts as a powerful reagent for the detection of IgG.

P3296-5Ml′s updated product number is GE17-0618-01

Aplicação

Protein G-Sepharose™ is used in affinity chromatography, protein chromatography, antibody purification and characterization, immunoaffinity matrices, protein A, G and L resins, protein interaction, and purification and detection. Protein G-Sepharose™ has been used to develop a strategy to confirm the presence of anti-erythropoietin neutralizing antibodies in human serum as well as to compare methods for depletion of albumin and IgG from equine serum.

forma física

Suspension in 20% ethanol

Nota de preparo

Prepared with recombinant streptococcal Protein G from which the albumin-binding region has been genetically deleted

Informações legais

Sepharose is a trademark of Cytiva

produto relacionado

Nº do produto
Descrição
Preços

Pictogramas

Flame
GHS02

Palavra indicadora

Warning

Frases de perigo

H226

Declarações de precaução

P210 - P233 - P240 - P241 - P242 - P243

Classificações de perigo

Flam. Liq. 3

Código de classe de armazenamento

3 - Flammable liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

115.0 °F - closed cup

Ponto de fulgor (°C)

46.1 °C - closed cup

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Os clientes também visualizaram

Slide 1 of 5

1 of 5

Protein A–Sepharose™ 6MB aqueous ethanol suspension

Millipore

P6649

Protein A–Sepharose™ 6MB

Protein G recombinant from Escherichia coli, ≥90%

Sigma-Aldrich

19459

Protein G recombinant

Protein L–Agarose from Peptostreptococcus magnus recombinant, expressed in E. coli

Millipore

P3351

Protein L–Agarose from Peptostreptococcus magnus

Protein A-Sepharose™ CL-4B Cytiva 17-0780-01, pack of 1.5 g

GE17-0780-01

Protein A-Sepharose™ CL-4B

Protein G Mag Sepharose™ Cytiva 28-9440-08, pack of 1 × 500 μL

GE28-9440-08

Protein G Mag Sepharose™

Elena Kotova et al.
PLoS genetics, 5(2), e1000387-e1000387 (2009-02-21)
Recently, the nuclear protein known as Poly (ADP-ribose) Polymerase1 (PARP1) was shown to play a key role in regulating transcription of a number of genes and controlling the nuclear sub-organelle nucleolus. PARP1 enzyme is known to catalyze the transfer of
Cordula M Stover et al.
Journal of immunology (Baltimore, Md. : 1950), 180(5), 3313-3318 (2008-02-23)
Properdin is a positive regulator of complement activation so far known to be instrumental in the survival of infections with certain serotypes of Neisseria meningitidis. We have generated a fully backcrossed properdin-deficient mouse line by conventional gene-specific targeting. In vitro
Cristina Hidalgo-Carcedo et al.
Nature cell biology, 13(1), 49-58 (2010-12-21)
Collective cell migration occurs in a range of contexts: cancer cells frequently invade in cohorts while retaining cell-cell junctions. Here we show that collective invasion by cancer cells depends on decreasing actomyosin contractility at sites of cell-cell contact. When actomyosin
Debby Kruijsen et al.
Journal of immunology (Baltimore, Md. : 1950), 185(11), 6489-6498 (2010-10-26)
Following infection with respiratory syncytial virus (RSV), reinfection in healthy individuals is common and presumably due to ineffective memory T cell responses. In peripheral blood of healthy adults, a higher CD4(+)/CD8(+) memory T cell ratio was observed compared with the
Aaron Pinnola et al.
The Journal of biological chemistry, 282(44), 32511-32519 (2007-09-11)
Poly(ADP-ribose) polymerase 1 protein (PARP1) mediates chromatin loosening and activates the transcription of inducible genes, but the mechanism of PARP1 regulation in chromatin is poorly understood. We have found that PARP1 interaction with chromatin is dynamic and that PARP1 is
Exibir todos os documentos relacionados

Protocolos

Immunoprecipitation Procedure

To determine the molecular weights of protein antigens, to study protein/protein interactions, to determine specific enzymatic activity, to monitor protein post-translational modifications and to determine the presence and quantity of proteins.

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica