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Key Documents

48900

Sigma-Aldrich

Giemsa Stain, Modified Solution

(for the staining (of cellular blood components and blood parasites))

Sinônimo(s):

Azure eosin methylene blue, Giemsa solution

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About This Item

Número CAS:
Número CE:
Número MDL:
Código UNSPSC:
41116121
ID de substância PubChem:
NACRES:
NA.25

tipo

(for research use only)

Nível de qualidade

prazo de validade

limited shelf life

técnica(s)

microbe id | staining: suitable

adequação

Chlamydia spp.
Histoplasma spp.
Plasmodium spp.
Trichomonas spp.
bacteria
protozoa
spirochetes

cadeia de caracteres SMILES

[Cl-].CN(C)c1ccc2N=C3C=CC(=[NH2+])C=C3Sc2c1

InChI

1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H

chave InChI

NALREUIWICQLPS-UHFFFAOYSA-N

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Aplicação

Tested for staining blood smears according to G. Clark (ed.), Staining Procedures, 4th edition, Williams & Wilkins, 173 (1981); together with May-Grünwald Solution for pap staining according to Pappenheim.

Atenção

store at 20°C

Princípio

When blood films are stained using Giemsa Stain, the nucleus and cytoplasm of white blood cells take on characteristic blue or pink coloration. The use of purified eosin and thiazine dyes minimizes lot-to-lot variation.

forma física

Solution of azure B/azure II-eosin/methylene blue 1:12:2 (w/w/w) in glycerol/methanol 5:24 (v/v); total dye content: 0.6 % (w/w)

Reconstituição

Fix 10 min. in methanol; air-dry; immerse 45 min. in Giemsa Solution; rinse with distilled water; air-dry

Palavra indicadora

Danger

Classificações de perigo

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1

Órgãos-alvo

Eyes

Código de classe de armazenamento

3 - Flammable liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

53.6 °F

Ponto de fulgor (°C)

12 °C

Equipamento de proteção individual

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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The Journal of cell biology, 219(11) (2020-09-05)
The mechanisms by which TP53, the most frequently mutated gene in human cancer, suppresses tumorigenesis remain unclear. p53 modulates various cellular processes, such as apoptosis and proliferation, which has led to distinct cellular mechanisms being proposed for p53-mediated tumor suppression
Tobias Koeniger et al.
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Although the bone marrow contains most hematopoietic activity during adulthood, hematopoietic stem and progenitor cells can be recovered from various extramedullary sites. Cells with hematopoietic progenitor properties have even been reported in the adult brain under steady-state conditions, but their
Tomasz Dobrzycki et al.
Communications biology, 3(1), 71-71 (2020-02-15)
Gata2 is a key transcription factor required to generate Haematopoietic Stem and Progenitor Cells (HSPCs) from haemogenic endothelium (HE); misexpression of Gata2 leads to haematopoietic disorders. Here we deleted a conserved enhancer (i4 enhancer) driving pan-endothelial expression of the zebrafish
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American journal of cancer research, 10(9), 3047-3060 (2020-10-13)
DNA methyltransferase 1 (DNMT1) is scientifically validated as a molecular target to treat chemo-resistant pancreatic ductal adenocarcinoma (PDAC). Results of clinical studies of the pyrimidine nucleoside analog decitabine to target DNMT1 in PDAC have, however, disappointed. One reason is high

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