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Key Documents

56822

Sigma-Aldrich

Immersion oil

for microscopy

Sinônimo(s):

immersion medium for light microscopy

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About This Item

Número MDL:
MFCD00131369
Código UNSPSC:
12171500
NACRES:
NA.47

fonte biológica

synthetic

grau

for microscopy

forma

liquid

índice de refração

n20/D 1.516

viscosidade

100-120 mPa.s(20 °C)

densidade

1.025 g/mL at 20 °C

aplicação(ões)

hematology
histology

temperatura de armazenamento

room temp

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Descrição geral

Immersion oil is a clear, viscous liquid with optimized refractive properties, specifically modified to closely approximate the refractive index (RI) of glass (ne = 1.5). It is used in conjunction with an objective lens to enhance the resolving power.

Aplicação

Immersion oil is used for high-resolution (1000X) light microscopy work in conjunction with an oil immersion objective lens to optimize microscopic examinations of histological, cytological, hematological, and bacterial specimen material after it has been fixed, embedded, stained, or counterstained, and mounted.

Princípio

Immersion oil is applied dropwise to stained and mounted or non-mounted specimen material to form a clear film between the specimen and the microscope lens to eliminate the deflection of incident light and thus substantially enhance the optical efficiency of the lens.

Pictogramas

Environment
GHS09

Palavra indicadora

Warning

Frases de perigo

H410

Declarações de precaução

P273 - P391 - P501

Classificações de perigo

Aquatic Acute 1 - Aquatic Chronic 2

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Certificados de análise (COA)

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Theis Sommer et al.
Scientific reports, 8(1), 13104-13104 (2018-09-01)
The catalytic mechanism of the cyclic amidohydrolase isatin hydrolase depends on a catalytically active manganese in the substrate-binding pocket. The Mn2+ ion is bound by a motif also present in other metal dependent hydrolases like the bacterial kynurenine formamidase. The
P L Appleton et al.
Journal of microscopy, 234(2), 196-204 (2009-04-29)
Visualizing overall tissue architecture in three dimensions is fundamental for validating and integrating biochemical, cell biological and visual data from less complex systems such as cultured cells. Here, we describe a method to generate high-resolution three-dimensional image data of intact
Thomas P Burghardt et al.
Applied optics, 48(32), 6120-6131 (2009-11-12)
Total internal reflection fluorescence (TIRF) microscopy uses the evanescent field on the aqueous side of a glass/aqueous interface to selectively illuminate fluorophores within approximately 100 nm of the interface. Applications of the method include epi-illumination TIRF, where the exciting light

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